IndraLab

Statements

OTUB1 binds FOXM1. 50 / 55
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"Through a review of the literature, we found that OTUB1 binds to FOXM1 and reduces the ubiquitination level of FOXM1 [ 23 , 24 ]."
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"Karunarathna U et al. demonstrated that OTUB1 can bind to and stabilize FOXM1 in epirubicin-treated breast cancer cells, which enhances its role in DNA damage response and epirubicin resistance of breast cancer cells [52]."
40003882
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"We hypothesized that LINC00857 may provide a scaffold for the interaction between OTUB1 and FOXM1 and that this scaffold increases FOXM1 deubiquitination."
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"We then set out to demonstrate the effect of this binding on the interaction between FOXM1 and OTUB1."
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"To this end, we first investigated the interaction between OTUB1 and FOXM1."
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"Collectively, these results suggest that LINC00857 may function as a scaffold that facilitates interactions between FOXM1 and OTUB1, thereby decreasing the rate of FOXM1 degradation.As p53 mutation is[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Through a review of the literature, we found that OTUB1 binds to FOXM1 and reduces the ubiquitination level of FOXM1 [ 23 , 24 ]."
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"However, FOXM1-OTUB1 interaction prevails, enhancing FOXM1 expression and conferring resistance ( xref )."
38847267
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"Importantly, our study confirmed that LINC00857 binds FOXM1 and OTUB1; moreover, we also found that knockdown of LINC00857 reduced the binding of FOXM1 to OTUB1, while overexpression increased this bi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Therefore, we believe that LINC00857 forms a protein scaffold that enhances the interaction between FOXM1 and OTUB1 and promotes the deubiquitination of FOXM1 by OTUB1."
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"Finally, a ChIRP experiment was used to confirm that LINC00857 specifically binds FOXM1 and OTUB1."
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"Mechanistically, we demonstrated that LINC00857 forms a protein scaffold that strengthens the interaction between OTUB1 and FOXM1."
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"Importantly, our study confirmed that LINC00857 binds FOXM1 and OTUB1; moreover, we also found that knockdown of LINC00857 reduced the binding of FOXM1 to OTUB1, while overexpression increased this bi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Therefore, we believe that LINC00857 forms a protein scaffold that enhances the interaction between FOXM1 and OTUB1 and promotes the deubiquitination of FOXM1 by OTUB1."
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"Mechanistically, we demonstrated that LINC00857 forms a protein scaffold that strengthens the interaction between OTUB1 and FOXM1."
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"LINC00857 upregulates FOXM1 expression and serves as a scaffold that enhances the interaction between FOXM1 and OTUB1, thus preventing deubiquitination of FOXM1, favoring FOXM1 accumulation, and leading to EMT and metastasis in pancreatic cancer."
37381723
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"OTUB1 interacts with FOXM1 in breast cancer cells."
26148240
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"In these cell extracts, we detected FOXM1 after immunoprecipitation with an OTUB1 antibody and vice versa, but not with the control IgG, indicating that OTUB1 interacts with FOXM1 either directly or as part of a larger complex."
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No evidence text available
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No evidence text available
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"Karunarathna U et al. demonstrated that OTUB1 can bind to and stabilize FOXM1 in epirubicin-treated breast cancer cells, which enhances its role in DNA damage response and epirubicin resistance of breast cancer cells [ xref ]."
40003882
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"In agreement, co-immunoprecipitation experiments demonstrated that FOXM1 expression is associated with OTUB1 binding but inversely correlates with conjugation to the protein degradation-associated Lys-48-linked ubiquitin-chains."
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"The FOXM1-OTUB1 interaction was further confirmed by the ability of FOXM1 to co-precipitate with a transiently transfected Flag-tagged OTUB1 ( xref )."
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"Epirubicin treatment caused a rapid decrease in the FOXM1-OTUB1 interaction in the sensitive MCF-7 cells but the levels of FOXM1-OTUB1 complexes were largely maintained or increased in the resistant MCF-7Epi R cells ( xref ; also see Figure 3)."
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"OTUB1 interacts with FOXM1 in breast cancer cells."
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"Given that OTUB1 is a deubiquitinating enzyme that removes polyubiquitin chains and promotes stability of target proteins, xref , xref , xref these data suggest that OTUB1 binds to FOXM1 and modulates its ubiquitination and degradation in response to genotoxic treatment in breast cancer."
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"We next examined whether OTUB1 binding is associated with the suppression of FOXM1 ubiquitination and downregulation in the epirubicin-sensitive and -resistant MCF-7 cells."
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"LINC00857 upregulates FOXM1 expression and serves as a scaffold that enhances the interaction between FOXM1 and OTUB1, thus preventing deubiquitination of FOXM1, favoring FOXM1 accumulation, and leading to EMT and metastasis in pancreatic cancer."
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No evidence text available
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"The result showed that epirubicin treatment caused a prominent decrease in FOXM1 levels, which was associated with decreased interactions with OTUB1 in the sensitive MCF-7 cells."
26148240
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"This induction in Lys48-linked polyubiquitination by epirubicin in MCF-7 cells was also associated with a decline in the FOXM1-OTUB1 interaction and FOXM1 expression in the MCF-7 cells."
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"In PC, LINC00857 acts as a protein scaffold to bind FOXM1 to ovarian tumor family deubiquitinase ubiquitin aldehyde binding 1 (OTUB1), thereby inhibiting FOXM1 degradation through the ubiquitin-proteasome pathway xref ."
38725864
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"These results are consistent with the notion that OTUB1 binds to FOXM1 and limits its ubiquitination and degradation in MCF-7 breast cancer cells."
26148240
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"We then set out to demonstrate the effect of this binding on the interaction between FOXM1 and OTUB1."
36272615
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"The fact that OTUB1 interacts with FOXM1 also supports that FOXM1 deubiquitination occurs through a catalytic mechanism, that requires interactions with OTUB1."
26148240
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No evidence text available
36272615
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No evidence text available
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27167337
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"To this end, we first investigated the interaction between OTUB1 and FOXM1."
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No evidence text available
27167337
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"To confirm the mass spectrometry based proteomic finding, we first examined whether endogenous OTUB1 binds to FOXM1 in the drug sensitive MCF-7 and the drug resistant MCF-7Epi R cells by co-immunoprecipitation."
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"In these cell extracts, we detected FOXM1 after immunoprecipitation with an OTUB1 antibody and vice versa, but not with the control IgG, indicating that OTUB1 interacts with FOXM1 either directly or as part of a larger complex."
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"Given that OTUB1 is a deubiquitinating enzyme that removes polyubiquitin chains and promotes stability of target proteins, XREF_BIBR, XREF_BIBR, XREF_BIBR these data suggest that OTUB1 binds to FOXM1 and modulates its ubiquitination and degradation in response to genotoxic treatment in breast cancer."
26148240
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"These results are consistent with the notion that OTUB1 binds to FOXM1 and limits its ubiquitination and degradation in MCF-7 breast cancer cells."
26148240
sparser
"Collectively, these results suggest that LINC00857 may function as a scaffold that facilitates interactions between FOXM1 and OTUB1, thereby decreasing the rate of FOXM1 degradation."
36272615
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"The fact that OTUB1 interacts with FOXM1 also supports that FOXM1 deubiquitination occurs through a catalytic mechanism, that requires interactions with OTUB1."
26148240
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"The physiological relevance of the regulation of FOXM1 by OTUB1 is underscored by the strong and significant association between FOXM1 and OTUB1 in breast cancer patient samples."
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