IndraLab

Statements

TNS3 binds CALM1. 20 / 20
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"No CAM-S-CAM-TPP or S(CAM-TPP) 2 was formed without Trx1 or Na 2 S 2 (Fig.  xref )."
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"This anticipated increase in H 2 S was confirmed by trapping it as the diagnostic product S(CAM-TPP) 2 ."
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"On the contrary, the longer linker in the CAM-C14-TPP structure results in the inability of this compound to fit snugly in the space between the optimally bound CAM and TPP fragments."
33922611
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"Using procedure B to assess RS(S) n S – under these conditions showed minimal S(CAM-TPP) 2 formation, except when there was excess Na 2 S, consistent with its reaction with existing persulfides (Supplementary Fig.  xref )."
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"Here, we tested the abovementioned CAM-TPP derivatives on chemoresistant triple negative breast cancer (TNBC) cell models, as well as on corresponding CSC-like spheroid cells."
33922611
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"In the current work, we studied the effect of CAM-TPP derivatives on CSC-like TNBC cell models."
33922611
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"The preliminary results with CAM-C10-TPP showed that normal human fibroblasts were less affected by submicromolar concentrations of CAM-TPP derivatives than cancer cells ( xref )."
33922611
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"This trapping procedure will also convert H 2 S into S(CAM-TPP) 2 ."
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"Thus, procedure A generates two products tagged with TPP for MS detection: CAM-S-CAM-TPP from the terminal S atom in RSS – and RS(S) n S – and S(CAM-TPP) 2 from H 2 S (Fig.  xref )."
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"Subsequent reduction with TCEP alongside trapping with excess IAM-TPP generates CAM-S-CAM-TPP from the terminal sulfur atom of RS(S) n S – and S(CAM-TPP) 2 from all the internal sulfane sulfur atoms RS(S) n S – (Fig.  xref , Supplementary Fig.  xref )."
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"The next step was to establish LC-MS/MS quantification of CAM-S-CAM-TPP and S(CAM-TPP) 2 ."
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"Na 2 S 2 treatment followed by analysis using procedure B also led to a dose-dependent formation of S(CAM-TPP) 2 consistent with PrS(S) n S – generation (Fig.  xref )."
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"Interestingly, S(CAM-TPP) 2 formation assessed by procedure B did not saturate, consistent with the reaction of initially formed PrSS – or PrS(S) n S – with Na 2 S 2 to form further PrS(S) n S – ."
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"This led to the detection of CAM-S-CAM-TPP by procedure A (Fig.  xref ), and this was prevented by the presence of ZnCl 2 which sequesters H 2 S preventing the formation of persulfides xref (Fig.  xref ) while S(CAM-TPP) 2 was not detectable by procedure B. Na 2 S alone did not generate CAM-S-CAM-TPP (Fig.  xref )."
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"Under these conditions there was also some polysulfide formation as assessed by procedure B (Supplementary Fig.  xref ) and S(CAM-TPP) 2 formation by procedure A consistent with H 2 S formation (Supplementary Fig.  xref )."
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"Detection of the terminal S required only procedure A. The H 2 S present in the initial homogenate was assessed by detection of S(CAM-TPP) 2 after protein precipitation in the first aliquot."
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"The bead-adsorbed proteins were then processed by procedure A to generate CAM-S-CAM-TPP (Fig.  xref ) and by procedure B to generate S(CAM-TPP) 2 (Fig.  xref )."
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"Snap-freezing the tissues during normoxia or ischaemia was followed by processing with IAM-TPP, enabling quantification of S(CAM-TPP) 2 and CAM-S-CAM-TPP."
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"Analysis of S(CAM-TPP) 2 and CAM-S-CAM-TPP again showed that the levels of H 2 S increased during ischaemia and decreased upon reperfusion (Fig.  xref )."
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"In addition, application of procedure B showed the generation of S(CAM-TPP) 2 (Fig.  xref ), consistent with detection of the internal sulfane sulfur polysulfide formation."
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