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"Then, we confirmed that OTUDin3 bound to OTUD3 and inhibited the deubiquitinating activity of OTUD3 by interfering with Ub binding to OTUD3."

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"The results showed that OTUDin3 bound to OTUD3 with a dissociation equilibrium constant (KD) value of 0.32 μM (Fig. 1J)."

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"This implied that OTUDin3 bound to OTUD3 with a certain specificity.Next, we performed in vitro deubiquitination assays to verify whether OTUDin3 inhibits the deubiquitinating activity of OTUD3."