IndraLab

Statements

OTUD1 increases the amount of MAP3K5. 6 / 6
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"As shown in Fig. 2d, ectopic OTUD1 expression upregulated apoptosis signal-regulation kinase-1 (ASK1) and increased ASK1 phosphorylation levels."
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"In addition, loss of OTUD1 reduced the c-Jun phosphorylation level in SKOV3, OVCAR8 and CAOV3 cells (Supplementary Fig. 2e), implying that OTUD1 probably regulates OSCS by activating the JNK/c-Jun pathway.Moreover, depletion of OTUD1 reduced the ASK1 phosphorylation level in SKOV3 and OVCAR8 cells (Supplementary Fig. 2f)."
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"As shown in Fig. 3b, ectopic expression of WT OTUD1 increased the ASK1 level, consistent with the observation that OTUD1 reduced ASK1 K48 ubiquitination."
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"Surprisingly, expression of the OTUD1 deubiquitinase-dead C320A mutant increased the ASK1 protein level to an extent comparable to that resulting from WT OTUD1 expression (Fig. 3b)."
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"As shown in Fig. 4g, after treating SKOV3 cells with doxorubicin (DOX) for 12 h, ectopic expression of OTUD1 but not Δ105–164 (M1) increased the phosphorylation levels of ASK1 at Thr838 and the expression levels of the downstream phosphorylated forms of JNK and c-Jun, all of which indicate ASK1 activation."
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"IHC analysis of xenograft tumors also revealed that OTUD1 but not OTUD1 increased ASK1 protein levels and JNK phosphorylation (Supplementary Fig. 5h)."
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