IndraLab

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TGFB1 decreases the amount of SDC4. 14 / 14
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"In addition, the results again indicated that siSmad3 with or without siSmad2 diminished the decrease in syndecan-4 core protein expression by TGF-beta 1 after a 24-h treatment."
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"These results suggest that the late downregulation of syndecan-4 expression by TGF-beta 1 is mediated by Smad2/3 at the mRNA level and by Smad3 at the protein level."
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"Recently, we found that TGF-beta 1 first upregulates and then downregulates the expression of syndecan-4, a transmembrane heparan sulfate proteoglycan, via the TGF-beta receptor ALK5 in the cells."
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"In this study, we observed that TGF-beta 1 first upregulates and then downregulates endothelial syndecan-4 expression."
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"Specifically, the downregulation of syndecan-4 expression by TGF-beta 1 is mediated by the interaction of Smad3 with Smad2 at the mRNA expression level, whereas only Smad3 is crucial at the core protein expression level."
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"The functional damage of vascular endothelial cells caused by the disturbed flow induces chronic TGF-beta 1 accumulation in the vascular wall, and the accumulated TGF-beta 1 suppresses the expression of syndecan-4 by activation of Smad2/3, which contributes to the progression of atherosclerosis [Tull et al., 2006; Nesbitt et al., 2009; Popovic et al., 2009]."
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"In other words, TGF-beta 1 induces endothelial biglycan synthesis; as a result, syndecan-4 expression is suppressed by enhanced TGF-beta 1 signaling."
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"Syndecan-4 mRNA expression was upregulated after 6h and downregulated after 24h by TGF-beta 1."
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"In contrast, SB203580 suppressed the early upregulation of syndecan-4 mRNA expression by TGF-beta 1, although the late downregulation was unaffected by the inhibitor."
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"The early upregulation and late downregulation of syndecan-4 mRNA expression by TGF-beta 1 were both not affected by siRNA mediated knockdown of Smad2."
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"On the other hand, siRNA mediated knockdown of Smad3 diminished the upregulation of syndecan-4 mRNA expression by TGF-beta 1, suggesting that Smad3 is a member of the signaling pathway that mediates the TGF-beta 1 -induced upregulation of syndecan-4 expression."
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"The late downregulation of syndecan-4 core protein expression by TGF-beta 1 was observed in cells treated with or without siRNA for Smad2, but this downregulation was reduced by siRNA for Smad3."
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"This result indicates that Smad3 is involved in the late downregulation of syndecan-4 core protein expression by TGF-beta 1."
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"These results indicate that the early upregulation of syndecan-4 mRNA expression by TGF-beta 1 is mediated by Smad3, whereas Smad2 or Smad3 alone is not involved in the late downregulation of syndecan-4 mRNA expression by TGF-beta 1; the late downregulation of syndecan-4 core protein expression by TGF-beta 1 is mediated by Smad3."
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