IndraLab

Statements

EGFP binds SCN9A. 5 / 5
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"The PCR product was digested with XhoI and BglII and ligated into the XhoI‐BamHI digested pN1‐EGFP expression plasmid (Clontech)."
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"As controls, cells were transfected with the empty pN1‐EGFP vector or mock transfected."
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"In some cases, the tat gene-encoding region within the minipolyprotein gene was replaced with either the eGFP gene (derived from pN1-EGFP; Clontech, Palo Alto, CA) or the Luc gene using the PCR amplif[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"As a control, cells were also transfected with the empty pN1‐EGFP vector (EGFP)."
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"To identify transfected cells all subunit combinations were co-transfected with 1 μg per plate of pN1-EGFP."
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