IndraLab

Statements

Ubiquitin binds USP50. 7 / 7
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"To test whether USP50 can bind Ub we expressed FLAG-USP50 and an I141R-FLAG-USP50 mutant along with Myc-tagged Ub (Myc-Ub) and performed FLAG immunoprecipitation."
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"By comparison, the mutant co-precipitated ~35% of the conjugates precipitated by the WT protein (Fig. 1B), suggesting that USP50 interaction with Ub conjugates is in part through its predicted Ub-binding face."
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"Using purified Ub, and bacterially expressed USP50, we found USP50 bound Ub chains but not free Ub (Supplementary Fig. 2A, B), suggesting a preference for conjugates.If USP50 has a role in replication, it might be expected to be associated with chromatin."
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"The Alphafold USP50:Ub structure predicts a complex similar to structures reported for USP domains with Ub (e.g., PDB: 3n3k xref )."
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"In the predicted USP50:Ub structure, Ub Ile44 is close to USP50 Ile-141 (Fig.  xref ), and leucine or isoleucine in USP50 is at this position in all 24 species (Supplementary Fig.  xref )."
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"To test whether USP50 can bind Ub we expressed FLAG-USP50 and an I141R-FLAG-USP50 mutant along with Myc-tagged Ub (Myc-Ub) and performed FLAG immunoprecipitation."
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"By comparison, the mutant co-precipitated ~35% of the conjugates precipitated by the WT protein (Fig.  xref ), suggesting that USP50 interaction with Ub conjugates is in part through its predicted Ub-binding face."
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