IndraLab

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OTUB2 binds RD21A. 7 / 7
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"To investigate this, we performed a yeast two‐hybrid (Y2H) assay, which confirmed that OTU2 interacts with RD21A but not with PbE3‐2 (Figure  xref ; Figure  xref )."
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"Consistent with these results, Split‐LUC assays demonstrated that OTU2 interacts with full‐length RD21A (RD21A), intermediate RD21A (iRD21A) and mature RD21A (mRD21A) (Figure  xref )."
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"To further validate the OTU2–RD21A interaction, we performed a co‐immunoprecipitation (Co‐IP) assay."
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"Additionally, BiFC assays demonstrated that OTU2 interacts with RD21A in both the cytoplasm and the cell membrane (Figure  xref ; Figure  xref )."
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"Together, these results confirm that OTU2 interacts with RD21A, highlighting its potential role in counteracting the PbE3‐2‐mediated degradation of RD21A."
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"To investigate the structural requirements for the interaction between OTU2 and RD21A, we used truncated derivatives of OTU2 in Y2H assays."
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"We found that RD21A interacts with OTUc (124–208 aa) but not with OTUn (11–123 aa), indicating that the C2H2 zinc finger domain is essential for the interaction between OTU2 and RD21A (Figure  xref )."
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