IndraLab

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"This may have resulted from two possibilities: (a) most of the MB molecules stayed in a tertiary structure which brought FAM and BHQ1 to be adjacent in space, rather than in a formation that separated FAM and BHQ1, in the absence of AFB1; (b) intermolecular hybridization occurred among MBs, which caused FAM from one MB molecule and BHQ1 from another MB molecule to come into a close, causing fluorescence quenching."

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"Recent MBs have been synthesized using a FAM reporter and two or three Dabcyl molecules as quenchers (XREF_FIG) at the 5 ' end [XREF_BIBR]."