IndraLab

Statements

USP39 binds GST. 7 / 7
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"The result showed that the K29-linked ubiquitination of Cyclin B1 was significantly reducible after incubated with GST-USP39 protein, suggesting that USP39 directly deubiquitinates Cyclin B1 ( xref d)."
37302347
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"Given that GST‐USP39 also exhibited slight binding affinity for other tested ubiquitins, we do not exclude the possibility that USP39 serves as a reader of Lys‐linked chains in cellulo ."
41408898
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"The pulldown assay further revealed that GST‐USP39 interacted most strongly with Dap20‐linked diUb, suggesting that USP39 might serve as its reader at least in vitro."
41408898
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"The eluate was sequentially incubated with GST-USP39 protein or GST protein followed by detection of K29 linked-CycB1 using anti-HA antibody with Western blotting."
37302347
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"To further investigate whether USP39 directly deubiquitinated CHK2, bacterially purified GST-USP39 and ubiquitinated CHK2 proteins were incubated together in a cell-free system."
30771428
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"We found that GST‐USP39 did not cleave Dap20‐linked diUb, whereas the pan‐deubiquitinase USP2cc readily cleaved it, suggesting that USP39 did not deubiquitinate at least Dap20‐linked ubiquitin chains (Figure  xref )."
41408898
sparser
"To test whether the Cyclin B1-USP39 interaction was direct, we performed GST-pull down assays using GST-USP39 and Flag-CycB1."
37302347