IndraLab

Statements

OTUD1 binds FlaG. 5 / 5
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"Indeed, peptides derived from AIFM1, CUL3, and PGAM5 were identified in FLAG-OTUD1 co-precipitates (Fig. xref , Supplementary Fig. xref )."
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"For the SEC23B half‐life assay, HEK293T cells were transfected with FLAG‐OTUD1 and treated with the protein synthesis inhibitor cycloheximide (Biorbyt, 100 µg mL −1 ) for the indicated time durations before collection."
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"Although the co-precipitation of endogenous AIFM1 with FLAG-OTUD1 was detected by the MS analysis (Fig. xref , Supplementary Fig. xref ), the co-precipitation of exogenous OTUD1 with AIFM1 was not detected (Supplementary Fig. xref )."
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"HL‐60 cells stably expressing Mock or OTUD1‐FLAG were treated with ATRA (1 µM) to induce differentiation of leukemic cells into granulocytes for 5 days and then stimulated by LPS (100 ng ml −1 ) for 4 h."
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"To further examine the physiological roles of OTUD1, proteins interacting with FLAG-OTUD1 in HEK293T cells were analyzed by immunoprecipitation-mass spectrometry."
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