IndraLab

Statements

SETD7 methylates OTUB1. 5 / 5
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"To further determine whether endogenous OTUB1 was methylated by SET7, we examined methylation of OTUB1 in SET7 +/+ and SET7 −/− H1299 cells after coimmunoprecipitation with anti-OTUB1 antibody."
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"When Flag-tagged WT OTUB1 (Flag-OTUB1) was cotransfected with empty vector, Myc-SET7 or its enzymatically deficient mutant (Myc-SET7-H297A) ( xref ) in OTUB1 −/− H1299 cells, monomethylation of OTUB1 in the cells transfected with WT SET7 was higher than in the cells transfected with empty vector control, while monomethylation of OTUB1 in the cells transfected with Myc-SET7-H297A was similar to the cells transfected with empty vector control, suggesting that OTUB1 might be methylated by endogenous SET7 in these cells or OUTB1 might be methylated at K122 by another unknown methyltransferase ( xref E )."
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"SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosis."
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"After searching the amino acid sequences of OTUB1 from different organisms, the ' KSK ' residues were identified in OTUB1 (Fig. 1A), in which lysine 122 might be methylated by SET7."
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"To further determine whether endogenous OTUB1 was methylated by SET7, we examined methylation of OTUB1 in SET7 and SET7 H1299 cells after coimmunoprecipitation with anti-OTUB1 antibody."
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