IndraLab

Statements

OTUD5 binds FlaG. 11 / 11
| 11
sparser
"At last, the PLA assay showed that FLAG-OTUD5 WT interacted with endogenous UBR5, measured by increased PLA spots, but the FLAG-OTUD5 ΔN17 mutant did not (Figure xref ), further supporting the conclusions."
30508113
sparser
"First, we found that expressing the FLAG-OTUD5 WT reversed (repressed) the YFP-MS2 reporter expression at the Fok1-induced DSB sites in OTUD5 knockdown cells (Figure xref )."
30508113
sparser
"To identify OTUD5 target(s) during NF-κB activation, we analyzed FLAG–OTUD5 associated protein identified by LC-MS."
40128189
sparser
"However, neither ΔN17 nor ΔUIM mutant were able to rescue the phenotype (Figure xref ; presence of FLAG-OTUD5 mutants at the spots indicate the normal expression and localization)."
30508113
sparser
"Below panel shows the expression confirmation of the FLAG-OTUD5 truncates."
30508113
sparser
"Consistently, overexpression of FLAG‐DUBA enhanced MYC‐DUBA expression in BV2 cells (Figure  xref ; Figure xref , Supporting Information)."
40755418
sparser
"The depleted UBR5 proteins were effectively rescued by expressing FLAG-UBR5 wild-type (WT), but not by a catalytically inactive FLAG-OTUD5 mutant (C224S) ( xref ) (Figure xref ), suggesting that the deubiquitinating activity of OTUD5 is necessary for preserving the UBR5 stability."
30508113
sparser
"Inactive mutation of D221 and C224 partially reduced expression levels of FLAG‐DUBA and MYC‐DUBA (Figure  xref ; Figure xref , Supporting Information), indicating that both D221 and C224 residues are required for the self‐deubiquitination of DUBA."
40755418
sparser
"Indeed, we have observed this in several ways; foci of endogenous OTUD5 and GFP-OTUD5 were detected at UVC-induced γH2AX spots (Figure xref and  xref , respectively; FLAG-OTUD5 in xref )."
30508113
sparser
"To test whether OTUD5 also interacts with SPT16 in an unbiased method, we performed FLAG-OTUD5 IP and GST-OTUD5 pulldown experiments from HeLa S3 cells and subjected the eluate to mass spec analysis (Figure xref )."
30508113
sparser
"A previous report showed that the DUB activity of DUBA is dependent on S177 phosphorylation. [ xref ] We also found that inactive mutation of S177 decreased the expression of FLAG‐DUBA and completely failed to increase MYC‐DUBA expression (Figure  xref ; Figure xref , Supporting Information)."
40755418