IndraLab
Statements
reach
"However, the affinity (K ) of OsOTUB1-cat towards Met1-diUb mutants was not almost unchanged or elevated, as seen in OTULIN, which was previously demonstrated to be activated by proximal Ub , but was significantly reduced: compared to OsOTUB1-cat with the Met1-diUb WT, the affinity (K ) of OsOTUB1-cat showed 1.45~1.58-fold, 1.52~1.91-fold, 3.65~4.30-fold and 4.20~6.23-fold reductions in the presence of Met1-diUb Q2H , Met1-diUb Q2N , Met1-diUb Q2A and Met1-diUb Q2E , respectively."
reach
"Hence, we reveal a mechanism of substrate assisted catalysis in which Glu16 of the proximal Ub activates the catalytic triad of OTULIN by both restricting the movement of the catalytic His339 and introducing a negative charge, presumably for the proper coordination of Asn341 for catalysis."