IndraLab

Statements

USP37 binds SND1. 11 / 11
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"As shown in xref , the treatment of SW480 cells with RO-3306 did not affect the interaction between USP37 and SND1."
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"To further explore the LC–MS/MS findings for USP37 in CRC, we conducted co-IP experiments to validate the interaction between endogenous USP37 and SND1."
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"GST-pull-down assays also confirmed this conclusion: a direct interaction exists between USP37 and SND1, and the DUB active site of USP37 is not key to their interaction ( xref I)."
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"To elucidate the interaction between USP37 and SND1 and establish its specific subcellular localization, we used immunofluorescence staining to visualize endogenous USP37 and SND1 in the CRC cell lines SW480, HCT116, and DLD1."
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"To map the minimal structural domains required for the SND1–USP37 interaction, we generated various truncated mutants of HA-USP37 and Myc-SND1 through two truncation approaches, narrowing down the binding region ( xref J and K, xref )."
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"We confirmed the direct interaction between USP37 and SND1, providing insights into the potential role of USP37 in regulating the stability of SND1."
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"To further confirm the interaction between exogenous USP37 and SND1, cells expressing Myc-SND1 and HA-USP37 were co-immunoprecipitated with anti-HA or anti-Myc antibodies."
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"We first investigated whether CDK1 regulates the interaction between USP37 and SND1."
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"Next, we sought to determine whether USP37 directly interacted with SND1."
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"These results unequivocally showed that exogenous USP37 formed a protein complex with exogenous SND1 ( xref F)."
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"The interaction between USP37 and SND1 was confirmed through extensive proteomics, ubiquitinomics, and interactomics, underscoring their synergistic effects on CRC proliferation and metastasis."
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