IndraLab

Statements

COPS6 binds MIF. 15 / 15
1 | 4 10
sparser
"For comparison of MIF–CSN6 with MIF–JAB1 binding, CoIPs of endogenous MIF–JAB1 complexes were performed from HEK293mycJAB1 cells by a similar protocol, except that the mouse monoclonal anti-JAB1 antib[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
sparser
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIF–CSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."
15757663
sparser
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIF–CSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
sparser
"Since the MIF–CSN6 interaction is somewhat weaker than that between MIF and JAB1/CSN5, it may be implied that JAB1 is the major interaction partner of MIF in the cell, but that additional weaker inter[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
reach
"The potential interaction between MIF and CSN6 was examined by CoIP between endogenous MIF and endogenous CSN6 using an anti-CSN6 antibody for CoIP."
15757663
reach
"Comparison with a CoIP of endogenous MIF–JAB1 complexes revealed that formation of the MIF–CSN6 complexes was slightly weaker than that of the MIF–JAB1 complexes."
15757663
reach
"Since CSN6 does not contain a functional JAMM motif within its MPN domain ( Fig. 4 A), endogenous formation of MIF–CSN6 complexes confirmed that the JAMM motif and JAMM-mediated isopeptidase activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
hprd
No evidence text available
15757663
reach
"35 S–MPN coimmunoprecipitated with rMIF was then revealed by SDS–PAGE electrophoresis in a 18% gel and, upon drying of the gel, exposure of an X-ray screen.Binding of MIF to CSN6 was examined by CoIP [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
sparser
"In summary, these data showed that the MPN domain independent of its JAMM function is sufficient to mediate MIF–JAB1 interaction in vitro and in vivo, that, on the side of MIF, sequence 50–65 accounts[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
sparser
"Binding of MIF to CSN6 was examined by CoIP analysis of endogenous complexes from HEK293 cells."
15757663
sparser
"To be able to directly compare the result of the MIF–CSN6 CoIP with a recently optimized CoIP of MIF with JAB1 from HEK293 cells expressing both endogenous JAB1 and ectopically overexpressed myc-tagge[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15757663
sparser
"This indicated that MIF–CSN6 protein complexes form intracellularly at physiological conditions and non-overexpressed endogenous concentrations of both components."
15757663
sparser
"At the same time, this analysis demonstrated that the MPN domain is sufficient in vivo to mediate MIF–JAB1 interaction and that a MIF–CSN6 interaction occurs in vivo."
15757663
sparser
"The potential interaction between MIF and CSN6 was examined by CoIP between endogenous MIF and endogenous CSN6 using an anti-CSN6 antibody for CoIP."
15757663