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USP13 binds RHBDF2. 18 / 18
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"Direct protein binding between USP13 and IRHOM2 was carried out via the GST pull-down analyses according to our previous reports methods13 24."
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"Mechanistically, we further determined that USP13 directly interacts with IRHOM2 and removes its K63-linked ubiquitination induced by ubiquitin-conjugating enzyme E2N (UBC13), a ubiquitin E2 conjugating enzyme, and thus prevents its activation of downstream cascade."
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"Also, the potential protein interaction of USP13 and IRHOM2 in mice were predicted and showed by GeneMANIA and STRING (Fig. S7B)."
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"Indeed, significant increase in ubiquitination of IRHOM2, and occurrence of protein binding of USP13 and IRHOM2 in corresponding vectors-transfected L02 cells were further confirmed by co-immunoprecipitation (Co-IP) detection ( xref )."
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"In light of the function of USP13 on regulation of IRHOM2-related signaling pathway, the in vivo and in vitro data forced us to put forward another crucial concerns by which whether the USP13 binds with IRHOM2 in the context of NASH pathologies."
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"The results actually indicate that USP13 directly binds with IRHOM2."
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No evidence text available
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sparser
"Also, the potential protein interaction of USP13 and IRHOM2 in mice were predicted and showed by GeneMANIA and STRING ( xref )."
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"Inactive rhomboid protein 2 (IRHOM2) was a crucial and positive regulator of inflammation-associated diseases and hepatocyte USP13 interacted with IRHOM2 and removed its K63-linked ubiquitination in response to metabolic stresses."
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"Thus, to identify potential inhibitory function of USP13 on Irhom2 signaling, we accordingly generated hepatocyte-specific Irhom2 -knockout (Hep Irhom2 KO) and hepatocyte-specific Usp13 and Irhom2 double deletion (Hep Usp13-Irhom2 DKO) mice (Supporting Information xref ), as indicated in Methods section."
36970206
sparser
"In light of the function of USP13 on regulation of IRHOM2-related signaling pathway, the in vivo and in vitro data forced us to put forward another crucial concerns by which whether the USP13 binds with IRHOM2 in the context of NASH pathologies."
36970206
sparser
"USP13 directly interacts with IRHOM2."
36970206
sparser
"Mechanistically, in response to metabolic stresses, USP13 directly interacts with IRHOM2 and removes its K63-linked ubiquitination induced by ubiquitin-conjugating enzyme E2N (UBC13), a ubiquitin E2 conjugating enzyme, and thus prevents its activation of downstream cascade pathway."
36970206
biogrid
No evidence text available
36970206
biogrid
No evidence text available
36970206
biogrid
No evidence text available
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sparser
"Liver USP13 interacts with IRHOM2 and restrains its ubiquitination by UBC13 and subsequent downstream signaling cascade activation, resulting in mitigation of NASH pathologies."
36970206
sparser
"The results actually indicate that USP13 directly binds with IRHOM2."
36970206