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USP43 binds HIF1A. 5 / 5
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"Importantly, the interaction between HIF-1α and USP43 did not prevent the formation of a HIF heterodimer, as HIF1-α and HIF1β still associated in USP43 null HeLa cells (Fig.  xref ), and neither USP43 depletion nor overexpression altered HIF-1α degradation kinetics using cycloheximide chase assay of HIF-1α degradation (Fig.  xref )."
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"Instead, we show that USP43 associates with HIF-1α and promotes the nuclear accumulation of HIF-1 and the chromatin binding of the transcriptional complex to specific HIF-1 target genes."
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"To understand how USP43 may facilitate a HIF-1 transcriptional response, we examined if USP43 associated with HIF-1α in hypoxia by incubating HeLa cells in 21 or 1% oxygen for 6 h and immunoprecipitating endogenous USP43 or HIF-1α."
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"USP43 bound to HIF-1α in hypoxia, and depletion of HIF1β did not prevent the association between endogenous USP43 and HIF-1α (Figs.  xref and  xref )."
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"The association of USP43 with HIF-1α was also observed in other cell lines (A549 and MCF7 cells) (Fig.  xref )."
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