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USP22 binds ZEB1. 14 / 16
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"In addition, HEK-293 cells were transfected with USP22 and ZEB1 expression plasmids as indicated and the interaction between USP22 and ZEB1 was conformed in Co-IP experiment (Supplementary Fig. S xref C)."
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"The results showed that only the N terminal of USP22 directly binds to ZEB1 (Fig. xref ), while the C terminal of USP22 may indirectly bind to ZEB1."
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"Through a co-IP assay, we found that USP22 can bind to ZEB1 mRNA and that knockdown of USP22 markedly facilitates the ubiquitination of ZEB1 and thus enhances its protein degradation."
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"Taken together, these data demonstrated that USP22 physically associates with ZEB1 in HCC cells."
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"Here, our results have demonstrated that USP22 interacts with ZEB1, and USP22/ZEB1 is recruited to the ZEB1-binding elements of VEGFA promoter region and USP22 decrease the accumulation of histone H2Bub."
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"Co-IP was used to validate the interaction between USP22 and ZEB1."
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"Having established that USP22 associates with ZEB1, and USP22/ZEB1 is recruited to the promoter of VEGFA in HCC cells, we thus turn to examine whether USP22 participates in maintenance of ZEB1 stability."
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"Next, we employed a Co-IP assay and found that USP22 interacts with ZEB1 ( xref )."
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"In addition, HEK-293 cells were transfected with USP22 and ZEB1 expression plasmids as indicated and the interaction between USP22 and ZEB1 was conformed in Co-IP experiment (Supplementary Fig. S3C)."
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"USP22 interacts with ZEB1, and USP22/ZEB1 is recruited to the ZEB1-binding elements of VEGFA promoter region in HCC cell lines."
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"The results demonstrated that the endogenous USP22 associates with ZEB1 in HCCLM3 and Huh7 cell lines (Fig. xref and Supplementary Fig. S xref A, B)."
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"We thus turned to ask whether USP22 could interact with ZEB1 in HCC cell lines."
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"Taken together, these data demonstrated that USP22 physically associates with ZEB1 in HCC cells.To examine whether USP22 was recruited to ZEB1-binding elements of VEGFA, we identified five potential ZEB1 binding sites on the promoter region of VEGFA based on the JASPAR database (Supplementary Fig. S4A) [45], and we named them as VEGFA-promoter I/II/III/IV/V (Fig. 3F)."
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"Co-IP was used to validate the interaction between USP22 and ZEB1."
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