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SPARC binds OTUD4. 14 / 14
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"Besides, the direct interaction between SPARC and OTUD4 was evidenced in the colonic tissues of DSS‐induced colitis mice (Figure xref , Supporting Information)."
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"The direct interaction between SPARC and OTUD4 was verified by co‐IP (Figure  xref )."
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"In order to better understand how SPARC‐OTUD4 interacts, we constructed a series of truncated fragments of SPARC and OTUD4 (Figure  xref )."
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"These results suggest that SPARC directly interacted with OTUD4."
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"Mechanistically, it is elucidated that SPARC competitively binds to OTUD4 in conjunction with MYD88, facilitating the translocation of p65 from the cytoplasm to the nucleus and subsequent activation of the p65-MLCK/MLC2 pathway, thereby compromising barrier integrity."
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"The direct interaction between SPARC and OTUD4 was verified by co‐IP (Figure 4D)."
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"Consequently, we speculated that the interaction between SPARC and OTUD4 may inhibit the binding of MYD88 to OTUD4, thereby activating the MYD88‐dependent signal transduction."
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"Besides, the direct interaction between SPARC and OTUD4 was evidenced in the colonic tissues of DSS‐induced colitis mice (Figure S5A, Supporting Information)."
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"Consequently, we speculated that the interaction between SPARC and OTUD4 may inhibit the binding of MYD88 to OTUD4, thereby activating the MYD88‐dependent signal transduction."
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"This suggests that SPARC can competitively bind to OTUD4, effectively obstructing the interaction between MYD88 and OTUD4."
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"This suggests that SPARC can competitively bind to OTUD4, effectively obstructing the interaction between MYD88 and OTUD4."
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"Mechanistically, we discovered that SPARC competitively binds OTUD4 with MYD88, leading to the translocation of P65 from cytoplasm to nucleus and activation of the P65‐MLCK/MLC2 pathway, ultimately disrupting barrier integrity."
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"Mechanistically, it is elucidated that SPARC competitively binds to OTUD4 in conjunction with MYD88, facilitating the translocation of p65 from the cytoplasm to the nucleus and subsequent activation of the p65‐MLCK/MLC2 pathway, thereby compromising barrier integrity."
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"To investigate the interaction between SPARC and OTUD4 proteins, Caco‐2 cells co‐transfected with the SPARC‐Flag plasmids and OTUD4‐Myc plasmids (MiaolingBio) were harvested using IP lysis buffer."
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