IndraLab

Statements

OTUB1 binds FlaG. 9 / 9
| 9
sparser
"However, expression of FLAG-OTUB1 stabilized UBE2E1 in OTUB1-depleted cells."
30282802
sparser
"The same results were also confirmed in T24 cells transfected with FLAG‐OTUB1 (Figure xref )."
33686769
sparser
"As shown in xref F , expression of a catalytic mutant, FLAG-OTUB1 C91S , restores levels of UBE2E1, whereas expression of OTUB1 T134R , fails to restore WT levels of UBE2E1 ( xref F )."
30282802
sparser
"The expression of siRNA-resistant FLAG-OTUB1 restores UBE2E1 protein levels when OTUB1 is knocked down ( xref C )."
30282802
sparser
"UBE2E1 protein levels could also be restored in OTUB1 CRISPR knockout cell lines by expressing either FLAG-OTUB1 or untagged OTUB1 ( xref D )."
30282802
sparser
"To examine the function of OTUB1 in bladder cancer cells, EJ cells were transfected with a FLAG‐OTUB1 (WT OTUB1) plasmid or FLAG‐OTUB1 C91S (catalytic‐inactive mutant) plasmid and OTUB1 expression was detected using western blotting (Figure xref )."
33686769
sparser
"To further test whether OTUB1 stabilizes UBE2E1, we overexpressed FLAG-OTUB1 in U2OS cells and probed for UBE2E1."
30282802
sparser
"We first cotransfected T24 cells with FLAG‐OTUB1 and HA‐ATF6 and detected ATF6 protein levels using western blotting."
33686769
sparser
"To confirm that OTUB1 directly regulates the stability of UBE2E1, we generated stable cell lines expressing siRNA-resistant FLAG-OTUB1 and knocked down endogenous OTUB1 by siRNA."
30282802