IndraLab

Statements

CANX binds PRNP. 10 / 12
| 2 8
sparser
"This possibility may be supported by the report that similarly to CypB, CNX interacts with PrP and reduces PrP-mediated toxicity (Wang et al., xref )."
28223916
reach
"The immunoprecipitation, confocal microscope and native polyacrylamide-gel electrophoresis results indicated that calnexin could bind PrP both in vitro and in vivo."
20506117
sparser
"Interaction of Prion Protein and Shadoo with the ER Chaperon Calnexin in the Lipid-Raft and Non-Raft Membrane Domains."
34940479
sparser
"Therefore, we were intrigued to examine whether this interaction of PrP and CNX is confined to raft- or non-raft-type membranes, and we wanted to test whether CNX is also a binding partner of Sho."
34940479
reach
"These results validate the mass spectrometry findings and show that GFP-PrP* retains its associations with factors of the early secretory pathway throughout its trafficking itinerary to the lysosome.Persistent CNX interaction with PrP* after induction of ER stress was particularly surprising given the earlier finding that this interaction is lost when PrP* exits the ER during stress (Satpute-Krishnan et al., 2014)."
31094677
sparser
"The immunoprecipitation, confocal microscope and native polyacrylamide-gel electrophoresis results indicated that calnexin could bind PrP both in vitro and in vivo."
20506117
sparser
"While intriguing in itself that ER chaperones attach to mature GPI-anchored proteins, it remains unknown, however, whether such an interaction of PrP and CNX is preferred in specific membrane domains versus others."
34940479
sparser
"For PrP, the Ni-NTA-bead pull-down experiments on the total lysates of PrP-EGFP cells confirm the presence of CNX in the bead-pulled PrP-containing eluates ( xref A), confirming that CNX may bind PrP, in line with earlier observations by Wang and coworkers [ xref ]."
34940479
sparser
"Next, we aimed to analyze if there is any preference for the interaction of prion proteins and calnexin depending on the type of membrane domains that the proteins reside in."
34940479
sparser
"We for the first time analyzed PrP-calnexin interaction."
20506117