IndraLab

Statements

NLRP3 binds USP13. 12 / 12
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sparser
"USP13 binds to the PYD of NLRP3, which prevents the interaction of NLRP3 with its E3 ubiquitin ligase TRIM31."
41004574
sparser
"In response to LPS treatment, the interaction between USP13 and NLRP3 rapidly increases in the early phase, while the interaction between TRIM31 and NLRP3 potently increases at relatively later time points."
41004574
sparser
"Co-IP assays showed that NLRP3 and USP13 interacted with each other (Fig.  xref f, g)."
33243234
biogrid
No evidence text available
33243234
sparser
"USP13 only binds to NLRP3 but not to ASC or pro–caspase-1, the other two subunits of the NLRP3 inflammasome, when coexpressed together in HEK293T cells ( xref )."
41004574
sparser
"In murine BMDMs, LPS priming induced a rapid binding of USP13 to NLRP3 but a relatively later binding of TRIM31 to NLRP3; however, USP13 deficiency resulted in an early and robust interaction between TRIM31 and NLRP3 ( xref )."
41004574
sparser
"The results showed that the truncated USP13 lacking the N-terminal region (amino acid residues 625 to 863) almost lost its ability to interact with NLRP3, indicating that NLRP3 binds to the N-terminal region of USP13 ( xref )."
41004574
sparser
"Moreover, the domains of USP13 required for USP13-NLRP3 interaction and USP13-Paxillin association were assessed by constructing and analyzing plasmids encoding for WT USP13(1–863) and four truncated proteins (Fig.  xref i)."
33243234
sparser
"Like WT USP13(1–863), USP13(301–863) and USP13(625–863) interacted with NLRP3, but USP13(1–300) or USP13(301–624) failed to interact with NLRP3 (Fig.  xref j), indicating that 625aa–863aa of USP13 are involved in USP13-NLRP3 interaction."
33243234
sparser
"We first confirmed the interaction between NLRP3 and USP13 in human embryonic kidney (HEK) 293T cells overexpressed with Myc-tagged human NLRP3 and Flag-tagged human USP13 by coimmunoprecipitation assay ( xref )."
41004574
sparser
"We have confirmed that USP13 binds to the PYD of NLRP3, which has been shown to be required for the binding of TRIM31 and FBXL2, two reported NLRP3 E3 ligases ( xref , xref )."
41004574
sparser
"Our results showed that USP13 binds to the PYD of NLRP3 protein and prevents NLRP3 K48-linked polyubiquitination on K192 and K496 residues, which in turn inhibits NLRP3 proteasomal degradation."
41004574