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EIF3F binds RABIF. 20 / 23
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"35 We showed here that the Mss4 protein amount not only increases during first stages of stress stimulation, but that Mss4 also efficiently binds the eIF3f protein."
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"On the basis of these results and the already published data, we hypothesize that Mss4 binds eIF3f avoiding its interaction with and phosphorylation by CDK11p46 (XREF_FIG)."
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"Molecular analysis of the Mss4-mediated inhibition of apoptosis showed that interaction of Mss4 with eIF3f (eukaryotic translation initiation factor 3 subunit f), a member of the translation initiation complex and a protein with distinct pro-apoptotic properties, is the critical event in the anti-apoptotic action of Mss4."
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"Under normal conditions, Mss4 is tightly bound to eIF3f, inhibiting its phosphorylation and subsequent association with eIF3 complex."
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"Molecular analysis of the Mss4 mediated inhibition of apoptosis showed that interaction of Mss4 with eIF3f (eukaryotic translation initiation factor 3 subunit f), a member of the translation initiation complex and a protein with distinct pro apoptotic properties, is the critical event in the anti-apoptotic action of Mss4."
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"Interaction of Mss4 with eIF3F prevents the eIF3F-mediated apoptosis."
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"Under normal conditions, Mss4 is tightly bound to eIF3f, inhibiting its phosphorylation and subsequent association with eIF3 complex."
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"To study whether stress application influences the binding of Mss4 to eIF3f, HEK293 cells were transfected with myc-tagged eIF3f along with GST-tagged Mss4 and stimulated for 8h with 5 mM H 2 O 2 or left untreated."
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"To study whether stress application influences the binding of Mss4 to eIF3f, HEK293 cells were transfected with myc tagged eIF3f along with GST tagged Mss4 and stimulated for 8h with 5 mM H 2 O 2 or left untreated."
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"The GST pull-down analysis showed that stress application strengthened the interaction between Mss4 and eIF3f ( xref , compare lanes 2 and 5)."
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"In agreement with these data, only cells stably overexpressing wt Mss4 but not the Mss4 F75A protein were able to withstand a long-lasting (16 h) hydrogen peroxide stress ( xref ), suggesting that binding of Mss4 to eIF3f is rather critical for escaping from apoptosis."
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"The association of Mss4 with eIF3f suggests that Mss4 is probably involved in regulation of protein translation."
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"The GST pull-down analysis showed that stress application strengthened the interaction between Mss4 and eIF3f (XREF_FIG, compare lanes 2 and 5)."
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"Moreover, enhanced expression of Mss4 in A7 melanoma cells neutralized the translation inhibitory effect of eIF3f, implying that association of eIF3f with Mss4 is of importance in protection of cells from apoptosis and that the anti-apoptotic properties of Mss4 are based on prevention of translation inhibition by eIF3f."
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"In agreement with these data, only cells stably overexpressing wt Mss4 but not the Mss4 F75A protein were able to withstand a long lasting (16h) hydrogen peroxide stress (XREF_FIG), suggesting that binding of Mss4 to eIF3f is rather critical for escaping from apoptosis."
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"On the basis of these results and the already published data, we hypothesize that Mss4 binds eIF3f avoiding its interaction with and phosphorylation by CDK11p46 ( xref )."
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"EIF3f also binds to the stress-stimulated protein MSS4, resulting in a reduced ability to inhibit protein synthesis [122] , and binds and stabilizes the MutS homolog hMSH4, a protein involved in doubl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To further investigate the significance of the Mss4 and eIF3f interaction in context of apoptosis, we transiently transfected A7 cells with plasmids coding for Mss4, eIF3f or for both and stimulated the cells 40h later for additional 8h with 5mM hydrogen peroxide and studied the cell morphology."
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"Finally, to substantiate the role of Mss4 and eIF3f interaction in protection of stress induced apoptosis, we transiently transfected divergent A7 melanoma cell lines that constitutively expressed different levels of either endogenous, recombinant wt or F75A mutated Mss4 proteins (XREF_FIG) with a plasmid coding for human eIF3f and subsequently subjected them to hydrogen peroxide stress for 8h."
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