IndraLab

Statements

USP6 binds CALM1. 14 / 14
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"By 3 days post agro-infiltration (dpai), only the leaves co-infiltrated with pCAM-USP6 and pUS-US71GFP showed strong GFP fluorescence ( xref B)."
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"Also, among various treatments, the leaves co-infiltrated with pUS-US71GFP and pCAM-USP6 showed the strongest green fluorescence ( xref B)."
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"The three constructs were individually co-infiltrated with pCAM-USP6 into N. benthamiana leaves."
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"Western blot assays showed a high amount of the GFP protein had accumulated in the leaves co-infiltrated with pCAM-USP6 and pUS-US71GFP."
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"PCAM-USP6 was co-infiltrated with these three bicistronic constructs into N. benthamiana leaves."
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"GFP fluorescence in the leaves co-infiltrated with pUS-US71GFP, pCAM-USP6, and pCAM-FveIF3g was much weaker than it was in the control leaves."
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"In contrast, a strong GFP fluorescence was observed in the pCAM-USP6 and US71GFP co-expressed leaves."
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"The results showed that strong GFP fluorescence was observed in the leaves co-infiltrated with pUS-US71GFP and pCAM-USP6, pUS-SY71GFP and pCAM-USP6, p35S-SY71GFP and pCAM-USP6, or pUS-Ca71GFP and pCAM-USP6 at 70 hpai."
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"Leaves co-infiltrated with pCAM-USP6 and an empty vector (pCAM-2300) were used as a negative control."
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"We then examined the effect of FveIF3g on P6 trans-activating activity by co-infiltrating N. benthamiana leaves with pUS-US71GFP, pCAM-USP6, and pCAM-FveIF3g or pUS-US71GFP, pCAM-USP6, and pCAM-2300 (control)."
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"Western blot results showed again that the expression level of the P6 protein was much lower in the leaves co-infiltrated with pUS-US71GFP, pCAM-USP6, and pCAM-FveIF3g compared with the control leaves ( xref D,E)."
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"All of the leaves co-infiltrated with pUS-US71GFP and pCAM-USP6, p35S-Ca71GFP and pCAM-USP6, or pSY-SY71GFP and pCAM-USP6 showed strong GFP fluorescence under the UV illumination by 3 dpai."
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"To determine which SVBV-encoded protein was a translation trans-activator, we cloned individual SVBV-US ORFs into the pCAM-2300 vector to produce pCAM-USP1, pCAM-USP2, pCAM-USP3, pCAM-USP4, pCAM-USP5, and pCAM-USP6."
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"Western blot assays showed that high levels of the P6 and the GFP proteins had accumulated in the leaves co-infiltrated with pCAM-USP6 and pUS-US71GFP, p35S-Ca71GFP, or pSY-SY71GFP."
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