IndraLab

Statements

OTUB1 increases the amount of SLC7A11. 10 / 10
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"Conversely, the deubiquitinating enzymes OTUB1 and ubiquitin specific peptidase 20 (USP20) mediate the deubiquitination of SLC7A11, thereby stabilizing SLC7A11 levels in cancer cells [6,122]."
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"Moreover, silencing OTUB1 reduced the level of endogenous SLC7A11."
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"Re-expression of OTUB1 restored SLC7A11 levels (Fig. 2D), indicating that OTUB1 regulates SLC7A11 expression.We subsequently assessed the levels of 4-HNE, a lipid peroxidation byproduct that served as a key marker of ferroptosis."
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"OTUB1 directly interacted with and stabilized SLC7A11; conversely, OTUB1 knockdown diminished SLC7A11 levels in cancer cells."
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"The depletion of endogenous OTUB1 reduces SLC7A11 expression and promotes ferroptosis in human cancer cells, which results in growth inhibition of human bladder cancer cell T24 mouse tumor xenografts."
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"OTUB1 inactivation promotes ferroptosis in human cancer cells primarily by down-regulating SLC7A11 levels."
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"Together, these data suggest that OTUB1 inactivation promotes ferroptosis in human cancer cells primarily by down-regulating SLC7A11 levels."
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"In addition, knockdown of OTUB1 diminished the HMW SLC7A11 complex formation and the overall levels of SLC7A11 and GSH in H522 cells (Fig. 5, F to H)."
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"Consistent with its ability to increase GSH, expression of WT OTUB1 increased SLC7A11 levels and its HMW complex observed under nonreducing conditions (fig."
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"In particular, our results show that (i) OTUB1 is a bona fide binding partner of SLC7A11 both in vitro and in vivo; (ii) OTUB1 acts as a major regulator for SLC7A11 activity in human cancer cells; (iii) OTUB1 inactivation promotes ferroptosis in human cancer cells primarily by down-regulating SLC7A11 levels; (iv) OTUB1 is overexpressed in human cancers and the OTUB1-SLC7A11 interaction is critical for tumor growth; (v) The OTUB1-SLC7A11 interaction is tightly regulated by CD44 in human cancer cells."
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