IndraLab

Statements

OTUB1 binds HIF. 13 / 13
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sparser
"We show evidence for an unexpected formation of a previously unknown strong, likely covalent, interaction between FIH and OTUB1."
31299612
sparser
"FIH and OTUB1 form a covalently linked protein complex."
31299612
sparser
"The input as well as the FLAG-specific IPs showed that beside N22 also C23 mutation abrogated both the HD and the non-covalent FIH:OTUB1 interaction, while mutations of S16 and/or S18 decreased the HD and the FIH:OTUB1 interaction by roughly 50% ( xref )."
31299612
sparser
"We next used the interaction of FIH with OTUB1 as additional positive control [ xref ] and confirmed this interaction in the case of OTUB1 WT in the interactome experiment and also subsequently by western blot analysis (Figs xref and xref )."
26752685
sparser
"FIH-dependent FIH-OTUB1 heterodimer formation is a hypoxia-regulated mechanism."
31299612
sparser
"The FIH-OTUB1 heterodimer forms co-translationally and is extraordinarily stable."
31299612
sparser
"This demonstrated that UBCH5B had the opposite effect on OTUB1 activity when OTUB1 was bound by FIH (forming the HT) than on monomeric OTUB1."
31299612
sparser
"These results show that OTUB1 maintains its enzymatic activity within the heterotrimeric complex with FIH, but the important regulation of its activity by the E2 enzyme UBCH5B is inverted, demonstrating a functional effect of FIH:FIH-OTUB1 HT formation."
31299612
sparser
"In order to determine whether the formation of FIH and OTUB1 heterodimers could affect OTUB1’s regulation on hypoxia signaling, we used FG4592 to stabilize HIF-1α under normoxia."
35732631
sparser
"Previously, we reported that FIH forms an oxomer with OTUB1. xref Here, we demonstrate that FIH forms a similar oxomer specifically with IκBβ."
38644795
sparser
"We observed that the formation of the FIH-OTUB1 heterodimer is slow (within the range of several hours) combined with slow degradation kinetics (up to 24 h)."
31299612
sparser
"Initially, we checked the effect of hypoxia on the formation of FIH and OTUB1 heterodimers."
35732631
sparser
"We previously showed that the cellular oxygen sensor factor-inhibiting HIF (FIH) interacts with OTUB1 [ xref , xref , xref , xref ] and hydroxylates asparagine 22, affecting OTUB1 substrate targeting and cellular energy metabolism [ xref ]."
35163456