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S binds EIF3F. 35 / 35
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"Meanwhile, parallel experiments confirmed that eIF3f could also interact with the S protein of another coronavirus, the avian coronavirus infectious bronchitis virus (IBV)."
18231581
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"Furthermore, eIF3f can also interact with the S protein of SARS-CoV and infectious bronchitis virus (IBV) to inhibit expression of host genes ( xref )."
29173589
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"Similar experiments were then performed to check if the full-length SARS-CoV S protein could also interact with eIF3f."
18231581
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"These results confirm that the full-length S protein can also interact with eIF3f."
18231581
sparser
"Interestingly, IBV-infected cells stably expressing a Flag-tagged eIF3f showed much higher translation of IL-6 and IL-8, suggesting that the interaction between coronavirus S protein and eIF3f plays a functional role in controlling the expression of host genes, especially genes that are induced during coronavirus infection cycles."
18231581
sparser
"We show here that eIF3f could interact with the S protein from two coronaviruses, SARS-CoV and the coronavirus infectious bronchitis virus (IBV)."
18231581
sparser
"Interaction of SARS-CoV S protein with eIF3f."
18231581
sparser
"Similar experiments were then performed to check if the full-length SARS-CoV S protein could also interact with eIF3f."
18231581
sparser
"The interaction between coronavirus S protein and eIF3f plays a functional role in controlling the expression of host genes, especially genes that are induced during coronavirus infection [ xref ]."
24740387
sparser
"These results confirm that the full-length S protein can also interact with eIF3f."
18231581
sparser
"The interaction between SARS-CoV S protein and eIF3f was further investigated by checking if the two proteins could form complexes in vitro."
18231581
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"We next set up to study the functional consequence of the confirmed interaction between SARS-CoV S protein and eIF3f in vitro."
18231581
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"Interaction of IBV S protein with eIF3f and inhibition of the translation of a reporter gene."
18231581
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"Co-immunoprecipitation was performed to check if IBV S protein could also interact with eIF3f."
18231581
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"Co-immunoprecipitation experiments were then carried out to test if IBV S protein could interact with the endogenous eIF3f in IBV infected cells."
18231581
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"The interaction between SARS-CoV S protein and eIF3f was further investigated by checking if the two proteins could form complexes in vitro."
18231581
sparser
"The authors suggested that the interaction between CoV S protein and eIF3f may play a functional role in controlling the expression of specific host genes, especially those that are induced by the viral infection."
27712623
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"We next set up to study the functional consequence of the confirmed interaction between SARS-CoV S protein and eIF3f in vitro."
18231581
reach
"Furthermore, eIF3f can also interact with the S protein of SARS-CoV and infectious bronchitis virus (IBV) to inhibit expression of host genes."
29173589
sparser
"These results confirm that the full-length IBV S protein could also interact with eIF3f."
18231581
sparser
"Co-immunoprecipitation experiments were then carried out to test if IBV S protein could interact with the endogenous eIF3f in IBV-infected cells."
18231581
sparser
"The inhibitory effect on translation rendered by the interaction between coronavirus S protein and eIF3f may account for the observed differential induction of virus-inducible genes at the transcriptional and translational levels."
18231581
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"Is the observed inhibition of translation by the interaction of coronavirus S protein with eIF3f beneficial to the virus?"
18231581
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"Co-immunoprecipitation was performed to check if IBV S protein could also interact with eIF3f."
18231581
reach
"These results confirm that the full-length IBV S protein could also interact with eIF3f."
18231581
reach
"Meanwhile, parallel experiments confirmed that eIF3f could also interact with the S protein of another coronavirus, the avian coronavirus infectious bronchitis virus (IBV)."
18231581
reach
"Interestingly, IBV infected cells stably expressing a Flag tagged eIF3f showed much higher translation of IL-6 and IL-8, suggesting that the interaction between coronavirus S protein and eIF3f plays a functional role in controlling the expression of host genes, especially genes that are induced during coronavirus infection cycles."
18231581
reach
"We show here that eIF3f could interact with the S protein from two coronaviruses, SARS-CoV and the coronavirus infectious bronchitis virus (IBV)."
18231581
sparser
"SARS-CoV S protein also interacts with the translation initiation factor eIF3f and inhibit host cell translation by preventing its nuclear import ( xref )."
33335518
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"The inhibitory effect on translation rendered by the interaction between coronavirus S protein and eIF3f may account for the observed differential induction of virus inducible genes at the transcriptional and translational levels."
18231581
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"However, immunofluorescent staining of cells expressing the two proteins clearly showed that the SARS-CoV S protein could interact with eIF3f and sequester the later to the site of S protein."
18231581
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"It is likely that eIF3f could gain access to the luminal side of the membranous compartments and interact with the S protein."
18231581
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"The authors suggested that the interaction between CoV S protein and eIF3f may play a functional role in controlling the expression of specific host genes, especially those that are induced by the viral infection.Using a yeast two-hybrid human protein library screen, Zhou et al. (2008) found that the SARS-CoV N protein binds to eukaryotic translation elongation factor 1 alpha (eEF1α), an integral part of the translational machinery; GTP-bound EF1alpha plays a key role in recruiting aminoacyl-tRNA to the A site of the 80S translation initiation complex during translation."
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"Our previous studies have demonstrated that the interaction between IBV S protein and eIF3f inhibits host cell protein translation, resulting in strong expression of the same genes at the transcriptional level, but only minimal to moderate induction at the translational level (Xiao et al., 2008)."
37058744
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"The interaction between coronavirus S protein and eIF3f plays a functional role in controlling the expression of host genes, especially genes that are induced during coronavirus infection [XREF_BIBR]."
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