IndraLab

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"Effective targeting and capturing of ectopically expressed full‐length FLAG‐USP16 and FLAGUSP36 was visualized by in‐gel fluorescence scanning followed by immunoblotting (Figure  xref , S8, Supporting Information)."

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"To validate our synthetic probes, we evaluated FUBI‐PA against the previously identified FUBI‐reactive proteases USP16 and USP36. [ xref ] To this end, we ectopically expressed full‐length FLAG‐USP16 and FLAGUSP36 in HEK293T cells (either WT or CA catalytic‐dead mutants) and performed cell lysate activity‐based labeling experiments."