IndraLab

Statements

CALM1 binds CYP. 41 / 41
| 41
sparser
"The authors classified this complex as heme oxy I [33] , which is spectrally similar to oxyferrous cytochrome P450-CAM [36] ."
20110129
sparser
"The hydroxyl group of T252 and WAT901 serves as hydrogen bond donors to the distal oxygen atom of the heme iron-bound dioxygen for wild-type P450-CAM ( Fig. 6 ) [1] ."
15752707
sparser
"It should be noted, however, that oxyferrous P450-CAM, a heme oxy I-type species, also shows a ferric–superoxide resonance Raman spectral characteristics (ν O–O = 1139–1147 cm −1 ) [38–41] ."
20110129
sparser
"Recombinant wild-type and T252A mutant P450-CAM proteins were expressed in Escherichia coli [18,30] and purified as reported [31] and stored at −70 °C at ∼100 μM concentrations in 100 mM potassium pho[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"This is because Mb has a neutral histidine as its proximal ligand, while in ferrous P450-CAM or CPO, the proximal ligands is a negatively charged cysteinate."
23639797
sparser
"Thus, we have first studied the binding of the various camphor analogs to ferric P450-CAM, whose spectral changes are generally more sensitive to differences in substrate-binding mode than those of th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"These spectral features are not seen or are negligible for the camphor- or camphor analog-bound wild-type (even at −40 °C) and T252A mutant (at 4 °C, but seen at −40 °C) enzymes and are likely to be a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The MCD spectrum of this species is shown in Fig. 4 A. The spectrum is very similar to that previously reported for low-spin ferric P450-CAM [20] and consists of a moderately intense derivative-shaped[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16197919
sparser
"To avoid appearance of such a P420-type spectral signal, it is important to prepare substrate-bound P450-CAM samples within 1 h after camphor removal at ∼4 °C. The MCD intensity of the derivative-shap[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The MCD spectrum of ferrous substrate-bound BMP at −55 °C had a sharp derivative-shaped component centered at 556 nm ( Fig. 4 A) that was likely due to a small amount of six-coordinate ferrous species[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16197919
sparser
"The addition of pre-cooled oxygen generated a stable oxyferrous BMP that had EA and MCD spectra that were similar to the corresponding spectra of oxyferrous P450-CAM ( Fig. 5 ) [11] ."
16197919
sparser
"The T252A P450-CAM mutant lacks the hydroxyl group of the T252 side chain that forms a hydrogen bond to the distal oxygen atom of the heme iron-bound dioxygen in the wild-type enzyme [1,30] ."
15752707
sparser
"Substrate (camphor) and its analogs (such as norcamphor and adamantanone) have also been shown to influence the IR spectra (CO stretching) of the ferrous–CO complex [43] and the resonance Raman spectr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"2 The ferric-NO complex of gsNOS ( Fig. 6 ) at 4 °C displays spectral properties that are comparable in band shape and peak positions when compared to those of the ferric-NO complex of the heme domai[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20110129
sparser
"The combination of low pterin-binding affinity, viscous cryosolvents, and low temperatures likely forces oxyferrous gsNOS into a heme oxy I conformation, whose spectral properties are similar to oxyfe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20110129
sparser
"PubMed, EMBASE® and the Cochrane Central Register of Controlled Trials (CCTR) were systematically reviewed for research articles pertaining to known CYP mediated CAM-drug interactions; case reports describing adverse events in patients, and clinical trials which examined the effects of herbs and supplements used during cancer treatment."
23625025
sparser
"Taking into consideration the few case reports and clinical trials in the literature describing adverse events associated with CAM-drug interactions, and the lack of human data to support CAM-CYP interactions, precautions regarding simultaneous use may be premature."
23625025
sparser
"The presence of a low spin (with water as the sixth ligand) component in substrate-bound ferric P450-CAM with camphor analogs (e.g., norcamphor) is generally correlated with less complementary binding[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The crystal structure of the (1 S )-camphor-bound ferric P450-CAM has been shown to contain two conformers, one having a hydrogen bond between the Tyr96 hydroxyl group and the 2-carbonyl group of the [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The overall UV–vis and MCD spectra of this adduct (Soret peak at 451 nm and a main band at 546 nm with a shoulder at ~ 575 nm) resemble those of the phosphine adducts of cysteinate-ligated ferrous P45[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23639797
sparser
"The similarity of the ferrous H93G(bis-THMP) complex to the phosphine adducts of ferrous P450-CAM as well as CPO is noteworthy."
23639797
sparser
"However, the derivative-shaped MCD Soret band of ferrous H93G(bis-THMP) Mb is 10 and 6 nm blue-shifted compared to those features in the spectra of the corresponding ferrous P450-CAM and CPO phosphine[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23639797
sparser
"Interestingly, the UV–vis and MCD spectra of six-coordinate ferrous phosphine bound H93G Mb show similarities to those of corresponding P450-CAM and CPO derivatives: i.e., ferrous H93G(bis-THMP) Mb is[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23639797
sparser
"In the camphor-bound ferric-cyanide complex of P450-CAM, which Schlichting and co-workers [46] have proposed as a good structural model for oxy-P450-CAM, the heme iron-coordinated cyanide indeed forms[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"CAM substances can also interact with conventional chemotherapies through their effects on CYP pathways that influence drug metabolism."
27528880
sparser
"Comparisons between the dioxygen complexes of wild-type and T252A mutant P450-CAM ( Figs."
15752707
sparser
"The ferric enzyme was first reduced by micro-liter additions of a near minimal amount of Na 2 S 2 O 4 (up to 0.5 mM from a 20 mg/mL stock) under N 2 at 4 °C to generate deoxyferrous P450-CAM, followed[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The crystal structure of camphor-bound ferric T252A P450-CAM mutant [30] has revealed that two water molecules (WAT687 and WAT720) exist inside the active site near Ala252 even in the ferric state."
15752707
sparser
"In addition, it appears that the significant loss of the camphor hydroxylation capability of the T252A P450-CAM mutant is not reflected in the structure or reactivity of its oxyferrous complex, but is[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"To each of the aqueous solutions of substrate-bound ferric P450-CAM thus prepared, ethylene glycol (1.5 volumes) containing appropriate amounts of the individual camphor analogs was added to give fina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"In particular, the results described herein demonstrate that the nature of the substrate can affect the physical properties (probed by spectroscopy) of oxyferrous P450-CAM, but does not affect signifi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"Crystallographic studies of cytochrome P450-CAM have shown that the positioning of the natural substrate (camphor) relative to the heme prosthetic group is very similar in the ferric, deoxyferrous, an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"With the rapid rates of product formation by bacterial P450 CAM and P450 BM3 , reduction of ferric P450 enzymes is highly dependent on the presence of substrate and can be correlated with substrate-in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22266245
sparser
"On the other hand, the wild-type ferric P450-CAM with bound (1 S )-camphor [19] , 5- exo -methoxycamphor ( Fig. S1A ), and norcamphor [37] are mixed spin complexes containing ∼80% (cf. ∼88% [38] ), ∼5[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"Equilibrium dissociation constants ( K d values) and spin state estimations (% high spin) of ferric P450-CAM complexes with these camphor analogs (5,5-difluorocamphor, not included, is expected to beh[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"Interestingly, unlike the case of wild-type ferric P450-CAM (∼100% high spin), the 5-methylenylcamphor complex of the ferric mutant is only ∼75% high spin, while the camphor adducts are almost totally[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"Dithionite reduction of substrate-free ferric (low spin) P450-CAM or of camphor- or camphor analog-bound ferric (high spin or mixed spin) P450-CAM in 60% (v/v) ethylene glycol under N 2 at 4 °C yielde[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"The shape and band pattern of the MCD spectra of the deoxyferrous P450-CAM derivatives do not vary with substrate (including the other camphor analogs, spectra of their enzyme complexes not shown), i.[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"3 Soret absorption peak positions are found to vary to some extent depending on substrate, solvent (0 or 60% ethylene glycol) as well as the T252A mutation for the deoxyferrous (408–411 nm) and oxyfer[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15752707
sparser
"Table II Inter-residue S···C/N interaction* Protein Cysteine Partner Name PDB code Residue Structure Atom Residue Structure (A) Main-chain partner Hemoglobin lBAB 105(A) H(9117) N M33 H(l2115) Ferredoxin Glycogen phosphorylase B Cytochrome P450-CAM lFDD lGPB 1PHB 11 495 85 T T s N D95 c W491 c G93 T I H(4/6) 148 E(2/3) N F150 H(1/4) Cyclophilin A Myohemerythrin Glutathione reductase Aconitase 2CPL 2MHR 3GRS 8ACN 161 35 333 440 178 257 E(6/8) N H(l7119) c G(4/4) c H(l/13) E(2/4) H(8110) N c c c K49 N43 A328 G437 Gl71 Gl72 S268 T H(3/24) E(3/3) s G(417) G(517) E(2/3) Lactate dehydrogenase 9LDT 187(A) E(l/2) N Gl77(A) H(l2/15) cc~ T306(B) (B) Side-chain partner Cytochrome P450-CAM 1PHB 85 s NE2 Q317 E(l/3) Tyrosine kinase transforming protein 1SHA 42 E(2/9) NH2 R32 E(4/5) Bovine papilloma virus <::ytochrome P450 2BOP 2HPD 327 62 E(l/7) NE R386 T CG N395 H(4110) s *Atom labels, according to the PDB convention, are given."
9669552
sparser
"3 Dithionite-reduction of substrate-free P450-CAM was considerably (>4-fold) slower than that of the substrate-bound enzyme (which was complete in ∼10 min with ∼0.25 mM dithionite)."
15752707