IndraLab

Statements


TP53 binds HBP1 and MDM2. 7 / 7
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sparser
"To determine whether the interaction between HBP1 and p53 or Mdm2 is a direct interaction, we conducted a GST pulldown assay with purified His-HBP1, His-p53, His-Mdm2, GST-HBP1, GST-p53, GST-Mdm2 proteins."

sparser
"As shown in xref B , the exogenous HBP1 also interacted with both exogenous p53 and Mdm2 in H1299 cells."

sparser
"In summary, it can be concluded that HBP1 directly interacts with both p53 and Mdm2 in vivo and in vitro ."

sparser
"The following reciprocal GST pulldown assay further testified to the interaction between HBP1 and p53 or Mdm2 in vitro ."

sparser
"As shown in xref A , the endogenous HBP1 interacted with both endogenous p53 and Mdm2 in A549 cells."

sparser
"As shown in xref B , overexpression of HBP1 disrupted the interaction between exogenous p53 and Mdm2 ( left panel ), whereas knockdown of HBP1 enhanced exogenous p53 interaction with Mdm2 ( center panel )."

sparser
"It has been found that HBP1 inhibits TCF4 transcriptional activation by interacting with TCF4 through its repression domain ( xref ), and because HBP1 interacts with the p53 binding domain of Mdm2, we assumed that HBP1 also inhibited the interaction of Mdm2 and p53 by its repression domain."