IndraLab

Statements

USP18 binds MAVS. 18 / 18
3 | 1 14
sparser
"The interaction between USP18 and MAVS is enhanced following viral infection, suggesting the possible regulation of MAVS activity by USP18."
34016972
sparser
"USP18 C64S , the mutation of cysteine residues in the enzymatic center of USP18, can interact with MAVS and enhances the ubiquitination as well as aggregation of MAVS comparable to the wild-type USP18, suggesting USP18 is involved in MAVS-mediated signaling pathway independent of its enzymatic activity."
34016972
sparser
"For example, USP18 specifically interacts with MAVS, promoting the K63-linked polyubiquitination and subsequent aggregation of MAVS when infected with SeV or Encephalomyocarditis virus (EMCV)."
36943869
sparser
"USP18 interacts with MAVS in the RLR signaling pathway."
34016972
sparser
"To investigate whether USP18 specifically interacts with MAVS, we utilized the Co-IP assay to examine the interaction between the signaling molecules in the RLR signaling pathway and USP18."
34016972
sparser
"The results showed that USP18 mainly interacted with MAVS, while there was no or weak association with upstream molecules RIG-I or MDA5 and downstream molecules TBK1 or IRF3 in the RLRs signaling pathway (Fig.  xref )."
34016972
biogrid
No evidence text available
34016972
biogrid
No evidence text available
34016972
sparser
"This enhanced complex formation of USP18 and MAVS was further validated through the PLA assay, in which the number of spots representing the endogenous USP18-MAVS complex increased significantly following SeV infection (Fig.  xref )."
34016972
sparser
"Since MAVS is localized in mitochondria, we further isolated the mitochondrial fraction and observed an enhanced interaction between MAVS and USP18 in mitochondria following SeV infection (Fig.  xref )."
34016972
sparser
"The elevated endogenous interaction between MAVS and USP18 was also confirmed in the RAW264.7 cells upon SeV infection (Fig.  xref )."
34016972
sparser
"There is evidence suggesting that USP18 can interact with MAVS and that this interaction promotes MAVS aggregation, resulting in enhancement of antiviral activity xref ."
37978268
reach
"The conclusion was supported by a series of experiments investigating the effects of different specific inhibitors of these events.There is evidence suggesting that USP18 can interact with MAVS and that this interaction promotes MAVS aggregation, resulting in enhancement of antiviral activity ."
37978268
sparser
"We observed that the interaction between MAVS and USP18 was not affected by this mutation (Fig.  xref )."
34016972
sparser
"The spatial colocalization and interaction between USP18 and MAVS were further confirmed by in situ proximity ligation assay (PLA), in which the number of red spots displaying the interaction between USP18 and MAVS."
34016972
biogrid
No evidence text available
34016972
sparser
"Here, we demonstrate that a mitochondria-localized deubiquitinase USP18 specifically interacts with MAVS, promotes K63-linked polyubiquitination and subsequent aggregation of MAVS."
34016972
sparser
"Notably, we also observed that the endogenous interaction between USP18 and MAVS was enhanced following the SeV infection in THP-1 cells (Fig.  xref ), indicating that USP18 may play a role in modulating the activity of MAVS."
34016972