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Ubiquitin activates USP5. 11 / 14
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"Indeed, Reyes-Turcu et al. [14] have demonstrated that binding of ubiquitin through its C-terminal Arg-Gly-Gly motif to the ZnF-UBP domain is sufficient to enhance USP5 catalytic activity in vitro ."
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"Moreover, substitutions of cysteine and histidine residues that coordinate the zinc ion ( Figure 2 c) interfere with ubiquitin-mediated USP5 activation."
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"Thus, USP5 activation is mediated by binding of the ubiquitin Arg-Gly-Gly motif to its ZnF-UBP ( Figure 3 a)."
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"Ubiquitin-associated domains (UBAs) in the C terminus indirectly contribute to the deubiquitinase activity of Usp5 by binding ubiquitin."
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"Moreover, addition of free ubiquitin to USP5 can further stimulate USP5 activity through binding to the ZnF-UBP domain via an as yet unknown mechanism [43] ."
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"It is known that occupancy of the S1 ' site by the proximal ubiquitin activates USP5 and that the C-terminal glycine (G76) of this ubiquitin is determinant for this activation XREF_BIBR XREF_BIBR."
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"Our finding suggests that the previous finding that ubiquitin (not C-terminal truncated) enhances the activity of USP5 37 is due to the binding of the beta-grasp domain of ubiquitin."
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"Recognition of the free C-terminus of the proximal ubiquitin in a poly-ubiquitin chain has been shown to stimulate the activity of USP5."
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"Both free and substrate conjugated polyubiquitin chains accumulate in leon mutants, suggesting that abnormal ubiquitin homeostasis caused tissue disorder and lethality in leon mutants."
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"While submicromolar concentrations of ubiquitin activate isopeptidase T, higher concentrations are inhibitory."
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"Similarly, alanine substitutions for most of the residues interacting with ubiquitin decrease (Asp264) or completely abolish (Trp209, Arg221 and Tyr261) USP5 activation by free ubiquitin [14] ."
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