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OTUD7B inhibits KDM1A. 3 / 3
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"OTUD7B or CoREST depletion only resulted in LSD1 degradation in p62 cells, but not in p62 cells (Figure 4E), suggesting that the binding to poly‐Ub chains on LSD1 is indispensable for p62‐mediated LSD1 degradation triggered by the deficiency of OTUD7B or CoREST.We next determined the type of poly‐Ub chains predisposed LSD1 to p62 binding."
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"We next generated OTUD7B reconstituting cell lines by expressing sgRNA‐resistant WT or mutant OTUD7B construct in MDA‐MB‐231 cells depleted of OTUD7B, and found only WT OTUD7B reconstitution rescued LSD1 decrease, excluding a possible off‐target issue during sgRNA‐mediated knockdown, also suggesting the DUB catalytic function of OTUD7B is required for its regulation of LSD1 (Figure 1F)."
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"The OTUD7B is responsible for the deubiquitination of LSD1 at the K226/277 residues, and OTUD7B depletion increases the LSD1 K63‐linked ubiquitination and p62‐mediated proteolysis [40]."
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