IndraLab

Statements

USP37 inhibits CMG. 7 / 7
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"Collectively, these results indicate that when forks experience stress in egg extracts and in mammalian cells, USP37 prevents CMG disassembly by TRAIP."
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"Strikingly, USP37 depletion enhanced CMG disassembly even when meDPCs were spaced ~1000 bp apart (Extended Data Fig. 6b, lanes 1–12)."
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"Since USP37 interaction with cohesin was reported previously , we tested whether immunodepletion of SMC3 phenocopies USP37 depletion in causing premature CMG unloading."
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"Notably, we have found that when replisome convergence was delayed by the TOP2 inhibitor ICRF-193, USP37 was required to suppress TRAIP-dependent premature CMG unloading."
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"Similarly, when we stalled forks with DNA-protein cross-links, USP37 prevented TRAIP-dependent CMG unloading."
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"Based on these observations, we propose that when converging forks stall, USP37 prevents CMG unloading by TRAIP (Fig. 6), and we speculate that a similar phenomenon occurs when mammalian cells are treated with topoisomerase inhibitors.We also found that TRAIP promotes CMG unloading in USP37-depleted extracts even when forks were stalled ~1 kb apart."
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"USP37 prevents CMG disassembly by TRAIP upon TOP2alpha inhibition."
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