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GAD1 binds CALM1. 17 / 17
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biogrid
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"In summary, in the Gad1–CaM1 complex, one CaM1 molecule attaches to two Gad1–CaMBDs of neighbouring subunits in a homohexameric enzyme, activating two adjacent active sites by relief of the correspond[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"This suggests that the two adjacent Lys residues are able to rescue each other's function when one of the two is mutated to Ala.In summary, K496 and K497 fulfil a role in modulating the equilibrium be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In the Gad1–CaM1 complex, the 415 nm-absorbing ketoenamine species is present over the entire pH range: CaM1, wrapped around two CaMBDs, keeps two AIDs away from the respective active sites."
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"CaM3 interacts with CRLK1 (calcium/calmodulin-regulated receptor-like kinase 1) and AGL24 (MADS box protein) whereas CaM1 interacts with GAD (glutamate decarboxylase 1), LP1 (lipid transfer protein), AK1 (aspartokinase) and HY5 (basic leucine zipper) protein ( xref ) [ xref , xref ]."
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"56 Synchrotron X-ray scattering data from solutions of Gad1 and from the Gad1–CaM1 complex were collected at the X33 beamline (DESY, Hamburg) using a MAR345 image plate detector."
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"64 The ab initio reconstructions were done with P 3 symmetry of hexameric Gad1 and of its complex with CaM1 introduced as a rigid constraint as well as without symmetry restrictions.The program BUNCH [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"68 The conformational space of Gad1–CaM1 complex was explored using the EOM approach, which takes flexibility into account by allowing for the coexistence of multiple conformations in solution."
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"Representative models of the Gad1–CaM1 complex were taken from the multiple rigid body modelling runs to yield an initial pool of models."
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"14 In contrast to that result, the NMR structure by Yap and co-workers, containing Gad CaMBD peptides bound to CaM, indicated a 1:2 complex, corresponding to a 1:3 stoichiometry for the Gad1–CaM1 comp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"30 We used SAXS to assess the structural organization of the Gad1–CaM1 complex and to validate the quaternary structure of Gad1 in solution."
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"Thus, the quaternary structure of Gad1 in solution does not differ from that in the crystal.The computed molecular mass ( MM ) of the Gad1–CaM1 complex given in Table 2 suggests a 1:3 stoichiometry fo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"This ambiguity was addressed by independent mass determination of the Gad1–CaM1 complex using size-exclusion chromatography coupled to a static light-scattering detector."
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"This yielded an MM of ∼383 kDa ( Table 2 ; Supplementary Data Fig. 4 ), in full accord with a 1:3 Gad1–CaM1 complex stoichiometry, which was further demonstrated by fluorimetric and UV-visible titrati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The UV-visible analysis shows a nearly identical behaviour.The values of the radius of gyration R g and maximum diameter D max increase significantly from Gad1 to the Gad1–CaM1 complex, indicating tha[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In all cases, multiple reconstructions were performed, which gave reproducible and consistent results.The low-resolution shape of the Gad1–CaM1 complex was reconstructed ab initio assuming P 3 symmetr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The CaM1–CaMBD complexes in BUNCH-generated models are slightly out of the plane perpendicular to the 3-fold axis, which may indicate high mobility of these peripheral portions.Further, the available [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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