IndraLab

Statements


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"Flag-USP15 significantly reduced p66Shc ubiquitin conjugation (Fig. xref )."

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"HA-Ub, Myc-p66Shc, and Flag-USP15 plasmids were co-transfected to HEK-293 cells."

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"This indicated that either the upregulation of USP15 and p66Shc, or their increased interaction or both contribute to liver I/R. Furthermore, Myc-p66Shc and Flag-USP15 plasmids were co-transfected to HEK293 cells in order to verify the physical association between USP15 and p66Shc."

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"HeLa cells overexpressed with Flag-USP15 were harvested by trypsinization after 36 h of transfection."

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"In addition, Flag-USP15 was co-precipitated efficiently by pS/pT antibody affinity purification ( Fig. 1 c), which further confirms the phosphorylation of USP15 in cells."

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"Labelling experiments were performed with HeLa cells lysate overexpressing Flag-USP5, Flag-USP7, Flag-USP15 or Flag-USP16 and their catalytically inactive versions ( xref )."