IndraLab

Statements

ALKBH5 increases the amount of USP22. 7 / 7
| 7
reach
"In addition, ALKBH5-mediated m6A methylation upregulates the expression of USP22 and RNF40, subsequently inhibiting the ubiquitination of histone H2A, promoting osteosarcoma growth and metastasis (16)."
37197432
reach
"Methyl RNA immunoprecipitation sequencing analysis and functional studies showed that ALKBH5 mediates its protumorigenic function by regulating m 6 A levels of histone deubiquitinase USP22 and the ubiquitin ligase RNF40."
35303054
reach
"Furthermore, ALKBH5-mediated m6A deficiency increases the expression of USP22 and RNF40 in osteosarcomas, promoting osteosarcoma cell growth and proliferation [40]."
40001461
reach
"Because ALKBH5 KD increased m A levels and significantly decreased USP22 mRNA steady-state levels, we performed mRNA stability analyses in scrambled siRNA and ALKBH5-silenced osteosarcoma cells."
35303054
reach
"ALKBH5 silencing led to significantly reduced expression of USP22 in 143B, U2OS, and OS-17 osteosarcoma cell lines (Fig. 4F; Supplementary Fig. S8D–S8G)."
35303054
reach
"Conversely, ALKBH5 overexpression increased USP22 expression in osteosarcoma cells (Fig. 4G; Supplementary Fig. S8H)."
35303054
reach
"Taken together, our data suggests that USP22 is highly sensitive to changes in m A levels and that ALKBH5 induces USP22 expression by maintaining an undermethylated state as increased methylation promotes accelerated decay of USP22 transcript in ALKBH5-depleted cells."
35303054