IndraLab

Statements


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"RD21A‐HA‐Ubn, ubiquitinated by PbE3‐2 in Escherichia coli using a previously established protocol, was incubated with GSTOTU2."

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"The ubiquitination level of RD21A‐HA‐Ubn was markedly reduced following incubation with GSTOTU2, whereas no reduction was observed with GST alone."

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"Incubation of recombinant GST-OTUB2 protein with OTUB2-IN-1 resulted in a significant inhibition of Ub-R110 cleavage by GST-OTUB2 in a dose-dependent manner (Supplementary Fig.  xref ), suggesting that OTUB2-IN-1 can inhibit the hydrolytic activity of recombinant GST-OTUB2 protein toward its substrate Ub-R110 in vitro."