IndraLab

Statements

USP49 binds Flag. 6 / 6
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"USP49 Flag-bound immunoprecipitates from these cells were resolved by SDS-PAGE and visualized by silver staining ( Fig. 4 A)."
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"Co-immunoprecipatation (co-IP) assay identified the interaction between USP49-BAG2 in 293T cells expressing Flag-USP49 and HA-BAG2 ( Fig. 4 D)."
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"To test this hypothesis, we overexpressed Flag-USP49 in 293T cells and analyzed the endogenous BAG2 protein levels."
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"Plasmids Flag-USP49, USP49 sh#1 and USP49 sh#2 were constructed and used in our previous study [ 13 ]."
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"For sample preparations, total proteins extracted from HCT116 cells with or without overexpression of Flag-USP49, were subjected to immunoprecipitation using antibodies against Flag-tag."
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"To identify the binding partners of USP49, we generated the HCT116 stable cell line ectopically expressing Flag-USP49."
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