IndraLab

Statements

Flag binds JOSD2. 5 / 5
| 5
sparser
"We next evaluated the binding by co-immunoprecipitation experiments via in vitro co-transfection of JOSD2-Flag with GFP-IMPDH2-HA plasmids or GFP-IMPDH2-K134R-HA plasmids in primary mouse peritoneal macrophages (MPMs)."
40177575
sparser
"To identify key substrate proteins interacting with JOSD2, anti-Flag magnetic beads were used to enrich Flag-JOSD2 protein and its interacting proteins in KYSE30 cells with exogenous Flag-JOSD2 overexpression."
38463028
sparser
"As indicated by the blue arrows in Figure xref , silver staining of the SDS-PAGE gel revealed numerous protein bands in the overexpression group but not in the control group, indicating that these proteins were specifically immunoprecipitated along with Flag-JOSD2."
38463028
sparser
"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids ( xref D and E)."
40177575
sparser
"The plasmid encoding GFP-IMPDH2-HA was simultaneously transfected with plasmids encoding Ub-Myc, JOSD2-Flag, or JOSD2-C24A-Flag into HEK293T cells. xref A demonstrates a significant decrease in the ubiquitination of IMPDH2 when JOSD2 was present."
40177575