IndraLab

Statements

Flag binds JOSD2. 8 / 8
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"To confirm the interaction between JOSD2 and PCNA, co-IP assays were conducted in 293T cells co-transfected with JOSD2-Flag and PCNA-HA plasmids."
40907659
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"To explore this hypothesis, 293T cells were cotransfected with PCNA-HA, Ub-Myc, and either JOSD2-Flag or JOSD2-C24A-Flag (a dominant-negative mutant of JOSD2), followed by treatment with MG132 to prevent proteasomal degradation."
40907659
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"To validate semi-endogenous binding, we overexpressed cGAS-Flag or JOSD2-Flag and performed Co-IP against their endogenous counterparts."
41351298
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"We next evaluated the binding by co-immunoprecipitation experiments via in vitro co-transfection of JOSD2-Flag with GFP-IMPDH2-HA plasmids or GFP-IMPDH2-K134R-HA plasmids in primary mouse peritoneal macrophages (MPMs)."
40177575
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"To identify key substrate proteins interacting with JOSD2, anti-Flag magnetic beads were used to enrich Flag-JOSD2 protein and its interacting proteins in KYSE30 cells with exogenous Flag-JOSD2 overexpression."
38463028
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"As indicated by the blue arrows in Figure xref , silver staining of the SDS-PAGE gel revealed numerous protein bands in the overexpression group but not in the control group, indicating that these proteins were specifically immunoprecipitated along with Flag-JOSD2."
38463028
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"Then, co-immunoprecipitation (Co-IP) and double immunofluorescence staining experiments further confirmed the interaction between JOSD2 and IMPDH2 via in vitro co-transfection of JOSD2-Flag and GFP-IMPDH2-HA plasmids ( xref D and E)."
40177575
sparser
"The plasmid encoding GFP-IMPDH2-HA was simultaneously transfected with plasmids encoding Ub-Myc, JOSD2-Flag, or JOSD2-C24A-Flag into HEK293T cells. xref A demonstrates a significant decrease in the ubiquitination of IMPDH2 when JOSD2 was present."
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