IndraLab

"By contrast, the experiments of V-ATPase activity and interaction with subunit H were conducted in vitro , where the compounds form direct contact with proteins."

"Mtb PtpA can prevent phagosome-lysosome fusion by dephosphorylating host protein VPS33B, and prevent phagosome acidification by binding to subunit H of the macrophage V-ATPase complex to block V-ATPase trafficking xref , xref ."

"Following this study, PtpA was also found to bind subunit H of the V-ATPase complex during infection [5] ."

"Although the function of these importin domains is not known, it has been suggested that they might mediate the interaction of subunit H with other V-ATPase subunits or with other cellular proteins ou[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Characterization of the Binding Interaction between JW-3 and the Subunit H of V-ATPase by Isothermal Titration Calorimetry Measurements."

"One of these strategies is the secretion of protein effectors that disrupt the macrophage’s innate antimicrobial defenses, such as protein-tyrosine phosphatase A (PtpA). xref , xref PtpA blocks phagosomal acidification and maturation by binding to subunit H of the macrophage V-ATPase complex and dephosphorylating VPS33B of the class C VPS macrophage complex. xref , xref Recent studies show that Mtb modulates host intracellular signaling xref , xref and that PtpA has a broader global effect on host cell signaling proteins. xref , xref , xref This, together with the promising properties of host kinase inhibitors as modulators of Mtb intracellular growth, xref , xref merits screening libraries of signaling inhibitors to identify associated cellular pathways that can be exploited as HDTs in the fight against TB."