IndraLab

"It has been shown that binding of CCL5 to SDC4 induces activation of PKCα , which is involved in multiple pathways including cell cycle progression, tumorigenesis and metastatic dissemination , but the mechanistic role of SDC4-CCL5 in ovarian cancer progression remains poorly understood."

"Taken together, these data indicate that some of the syndecan-4 molecules bound by RANTES were further oligomerized in the presence of the chemokine, and suggest that the syndecan-1 and syndecan-4 mol[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Moreover, we also observed in these conditions a shift of the respective apparent molecular masses of the PGs bound by RANTES, from 50–52 kDa to 45 kDa for RANTES bound syndecan-1, and from more than [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"The receptor pairs CXCL10-SDC4, IFNG-IFNGR1-IFNGR2, CCL5-SDC4 and CXCL16-CXCR6 were positively correlated with the therapeutic response to ICB (Fig. xref H)."

"To evaluate CCL5-SDC4 axis, we tested our model with recently published scRNA-seq data from 6 additional ovarian cancer patient-derived ascites (GSE146026)."

"Overall, NicheNet highlighted the CCL5-SDC4 axis as a potential mechanism for ovarian cancer cells to acquire anoikis resistance in ascites TME."

"Although our computational model does not consider the spatial proximity between the cells of interest, ascites from ovarian cancer patients creates a unique TME as a reservoir of pro-tumorigenic soluble factors including CCL5 and other cytokines reported in our previous work. xref In this study, we found that CCL5-SDC4 axis may play a crucial role in ovarian cancer cell survival and poor patient prognosis."

"To validate our model, we analyzed the recently published scRNA-seq study of malignant ascites from patients with advanced-stage ovarian cancer. xref Also this analysis highlighted the putative role of intercellular communication through CCL5-SDC4 axis in immune cells and ovarian cancer cells ( xref )."

"SDC4 is a heparan sulfate proteoglycan that modulates focal adhesion and has been implicated in cancer progression and metastasis. xref Elevated SDC4 expression has been reported in hepatocellular cancer and mesotheliomas. xref , xref Previous ovarian cancer study observed that SDC4 is expressed in normal ovary and in both benign and malignant ovarian tumors. xref CCL5 is elevated in various types of cancers, including ovarian cancer. xref It has been shown that binding of CCL5 to SDC4 induces activation of PKCα xref , xref , which is involved in multiple pathways including cell cycle progression, tumorigenesis and metastatic dissemination xref , but the mechanistic role of SDC4-CCL5 in ovarian cancer progression remains poorly understood."

"Our in vitro anoikis resistance analysis further supports the role of CCL5-SDC4 axis in ovarian cancer cell survival in ascites TME ( xref )."

"These interaction pairs include KLRD1-HLA-B, CD8A-LCK, KLRD1-HLA-E, CCL5-CXCR3, CCL5-SDC4, ADAM12-ITGB1 pairs, and are enriched in pathways such as regulation of immune response and cell-cell adhesion ( xref d)."

"We further analyzed the cell-cell adhesion pairs and found that the CCL5-SDC4 and ITGB1-ADAM12 ligand-receptor interactions contribute most in CD8-C6-LAYN and ADAM12 + CTLA4 + Tregs pairs, compared with other cell pairs ( xref e), further supporting the hypothesis that ADAM12 + CTLA4 + Tregs induce exhaustion of effector T cells through cell-cell adhesion by ADAM12-ITGB1 and SDC4-CCL5 interaction."

"In addition, we have encountered a negative association between CCL5 and GNA11, GNG13, and SDC4."

"Notably, distinct expression patterns of pairs like CCL5–SDC1, CCL5–SDC4, and CCL5–ACKR1 were observed across clusters of immune cells encompass various types, including B-cells, CD4+ T-cells, CD8+ T-cells, NK cells, and Tregs ( xref E)."

"Moreover, the binding of RANTES and CCL5 to SDC-1, SDC-4 and CD44 on HeLa cells was dependent on GAGs [10]."

"A study reported that CCL5 secreted by T cells can bind to the receptors SDC1 and SDC4 on the surface of tumor cells, thus promoting pancreatic tumor progression [33]."