IndraLab

Statements

USP15 binds HBx. 20 / 20
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sparser
"These results clearly indicate that HBx and USP15 interact specifically both in vitro and in vivo ."
28074857
reach
"To determine whether there is a possible association between HBx and USP15, perhaps under more physiologic condition, the CytoTrap yeast two-hybrid system was employed, and the results demonstrated that HBx could interact with USP15, and the region between HBx amino acid residues 51 and 80 is required for the interaction with USP15."
28074857
sparser
"USP15 binds to HBx and stabilizes HBx through removal of Lys 48 -linked polyubiquitin moieties from HBx, thus extending its half-life and increasing its steady-state level."
28074857
reach
"To further confirm the interaction between HBx and USP15 in vitro, the GST pull-down assay was performed."
28074857
sparser
"HBx associates with USP15 in vitro and in vivo ."
28074857
sparser
"This implied that the association of USP15 with HBx not only increases the stability of HBx but also augments HBx-mediated oncogenic signals."
28074857
sparser
"To further confirm the interaction between HBx and USP15 in vitro , the GST pull-down assay was performed."
28074857
reach
"We demonstrate for the first time that USP15 specifically interacts with HBx and consequently increases HBx stability and the steady-state level by inhibition of HBx ubiquitination and degradation."
28074857
sparser
"Given the observations that USP15 binds to HBx, trims ubiquitin from HBx, and consequently increases HBx stability and protein level, we went on to explore whether the transcriptional transactivation function of HBx could be increased as a result of elevated HBx protein level due to the interaction between USP15 and HBx."
28074857
sparser
"To determine whether there is a possible association between HBx and USP15, perhaps under more physiologic condition, the CytoTrap yeast two-hybrid system was employed, and the results demonstrated that HBx could interact with USP15, and the region between HBx amino acid residues 51 and 80 is required for the interaction with USP15."
28074857
sparser
"As shown in xref , GST and GST-HBx protein were well expressed, and 35 S-labeled USP15 was retained on the GST-HBx-conjugated sepharose whereas GST alone was not, indicating that USP15 could interact with HBx directly in vitro ."
28074857
sparser
"The physical and specific interaction between HBx and USP15 was confirmed from a series of comprehensive binding studies."
28074857
reach
"To assess the functional consequence of the interaction between USP15 and HBx, we first examined the effects of HBx on USP15 protein level and peptidase activities."
28074857
reach
"Given the observations that USP15 binds to HBx, trims ubiquitin from HBx, and consequently increases HBx stability and protein level, we went on to explore whether the transcriptional transactivation function of HBx could be increased as a result of elevated HBx protein level due to the interaction between USP15 and HBx."
28074857
sparser
"Furthermore, by using the GST pull-down assay, we demonstrated that HBx was able to bind to USP15 in the absence of a cellular context."
28074857
reach
"As shown in XREF_FIG, GST and GST-HBx protein were well expressed, and 35 S labeled USP15 was retained on the GST-HBx-conjugated sepharose whereas GST alone was not, indicating that USP15 could interact with HBx directly in vitro."
28074857
reach
"The physical and specific interaction between HBx and USP15 was confirmed from a series of comprehensive binding studies."
28074857
reach
"To assess the biological relevance of this interaction, HBx was transfected in Huh7 cells, and coimmunoprecipitation analyses showed that HBx interacted with endogenous USP15 and vice versa."
28074857
reach
"After replica plating, clones that grew on SD/galactose (-UL) but not on SD/glucose (-UL) plates at 37degreesC were defined as " positive " which indicates the interaction of USP15 and HBx or HBx deletion mutants."
28074857
sparser
"Taken together, these results strongly suggested that HBx and USP15 interact specifically both in vitro and in vivo ."
28074857