IndraLab

Statements

USP8 leads to the ubiquitination of SMO. 7 / 7
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"Inactivation of USP8 increases Smo ubiquitination and attenuates Hh induced Smo accumulation, leading to decreased Hh signaling activity."
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"As shown in XREF_FIG, inactivation of USP8 in S2 cells by RNAi dramatically enhanced the levels of Smo ubiquitination (XREF_FIG, lane 4, compare to lane 2, top panel), whereas the reduction of other DUBs by RNAi did not, suggesting that the inhibition of Smo accumulation by USP8 RNAi in wing discs was due to the increase in Smo ubiquitination."
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"We also provide evidence that the non visual beta-arrestin Kurtz (Krz) acts in parallel with Smo ubiquitination to control Smo cell surface expression, and that the deubiquitinating enzyme UBPY promotes Smo cell surface expression by counteracting Smo ubiquitination."
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"From this screen, we found that RNAi of the Drosophila UBPY and USP8 significantly increased the basal levels of Smo ubiquitination (XREF_SUPPLEMENTARY)."
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"We also show that inactivation of USP8 by RNAi or by overexpressing a dominant negative form of USP8 increases Smo ubiquitination in S2 cells and prevents Smo accumulation in wing discs."
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"Consistent with these data, the overexpression of USP8 reduced Smo SD123 ubiquitination, whereas the inactivation of USP8 increased Smo ubiquitination (XREF_FIG)."
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"We also found that inactivation of UBPY by RNAi increased Smo ubiquitination in the presence of Hh (XREF_FIG), suggesting that UBPY counteracts Smo ubiquitination in both Hh signaling " off " and " on " states."
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