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PRPF8 inhibits prp16-302 allele. 2 / 2
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"Furthermore, whereas second-step alleles improve the second step, the N-terminal alleles inhibit both catalytic steps, indicating that they act by different mechanisms.Alleles in three spliceosomal factors: U6, Cwc2, and Prp8, that suppress defects of the prp16-302 allele, identified important catalytic interactions stabilizing the first-step conformation but destabilized by the Prp16 ATPase at the transition to the second step."
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"These prp8 alleles significantly suppress growth defects of prp16-302 allele (Fig. 4B), and most of them (prp8-F331L, Y356C, Y590C/F, K603I, K611I/T, and S613T) also correct growth defects of prp16-R686I, demonstrating the generality of these effects (Fig. 4C)."
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