IndraLab

Statements

USP1 increases the amount of PCNA. 8 / 8
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"Expressing USP1 caused a decrease in PCNA-Ub levels, whereas USP1 expression did not affect PCNA ubiquitination levels, confirming that our experimental setup was functioning (Fig 3C)."
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"Instead, the fourth critical residue mutant (USP1 ) causes loss of almost all activity, comparable to USP , as judged by unchanged or accumulating levels of PCNA-Ub after doxycycline induction."
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"Downregulation of USP7 and USP1 increased the level of PCNA mono-ubiquitination induced by UV irradiation and oxidative stresses in different manners."
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"USP1 inhibition in sensitive cells results in aberrant accumulation of mono- and polyubiquitinated PCNA and reduced total PCNA levels."
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"These suggest that UBE2K has a previously unreported role in promoting polyubiquitination in the context of persistent PCNA monoubiquitination.We noted that USP1 inhibition reduced the levels of unmodified PCNA, which was restored upon RAD18 or UBE2K knockout (Fig. 3E)."
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"USP1 knockdown or ML-323 treatment reduced the expression of proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E1."
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"Proteomics analysis revealed that USP1 inhibition reduced total PCNA protein levels, whereas RAD18 or UBE2K knockout reversed this effect (Fig. 3F)."
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"Downregulation of USP1 increases the basal level of PCNA mono-ubiquitination, which is produced during DNA replication and after UV irradiation."
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