IndraLab

Statements


USP50 deubiquitinates PYCARD. 7 / 7
1 | 6

reach
"USP50 deubiquitinates the ASC protein."

"The deubiquitinating enzyme, ubiquitin-specific peptidase 50, regulates inflammasome activation by targeting the ASC adaptor protein"

reach
"However, the Flag-USP50 (C53S) mutant, did not affect polyubiquitination of the ASC protein, indicating that the Cys residue at position 53 is critical for the catalytic activity of USP50 deubiquitination of the ASC protein."

reach
"To further confirm our finding that USP50 deubiquitinates the ASC protein, we performed in vitro deubiquitination assays."

reach
"Ubiquitination of Flag-ASC proteins was significantly decreased in the presence of Flag-USP50 protein, indicating that USP50 directly deubiquitinates the polyubiquitination of the ASC protein."

reach
"Wild-type USP50 and the Flag-USP50 (H322A) mutant significantly deubiquitinated the ASC protein."

reach
"In conclusion, we here demonstrate a novel regulatory mechanism regarding the ASC protein which is deubiquitinated by USP50 and provide experimental evidence emphasizing the importance of a regulatory mechanism mediated by ubiquitination and deubiquitination in inflammasome activation."