IndraLab

Statements

USP4 binds MYC. 4 / 4
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"To whether USP4 can indeed deubiquitinate CENPF, HCT116 cells were transfected with CENPF-HA, Flag-UB, USP4-Myc or USP4 C311S-Myc, and then anti-HA immunoprecipitates were probed for the level of ubiquitination using Flag-antibody."
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"We then verified the specific interaction between USP4 and PPARα in HEK‐293T cells exogenously expressing Myc‐USP4 and Flag‐PPARα (Figure  xref )."
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"Immunoprecipitation with anti-Myc antibody demonstrated that Myc-USP4 interacted with overexpressed CENPF-HA (Fig. xref )."
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"To determine which type of PPARα ubiquitination is affected by USP4, we co‐transfected Myc‐USP4, Flag‐PPARα, and seven lysine‐specific ubiquitin mutants (K6, K11, K27, K29, K33, K48, and K63) into HEK‐293T cells."
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