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USP28 binds GST. 4 / 4
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"Briefly, purified GST-USP28 was incubated with histone H2A. The complex was then pulled down by GSH agarose beads, and the bound proteins were eluted with glutathione and analyzed by SDS-PAGE."
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"GST-USP28 was cloned into pGEX vector for expression in bacteria."
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"As shown in Fig. 4 C, both H2A and ub-K119-H2A were detected in the GST-USP28 pulldown complex, but not in the GST control complex, demonstrating that the interactions between USP28 and H2A is direct."
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sparser
"USP28 antibodies were generated by immunizing rabbits with a GST-USP28 fusion protein consisting of GST fused on its C terminus to the N-terminal 1–332 amino acids of USP28 and affinity purified (Beth[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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